BLIMP-1, A NOVEL ZINC FINGER-CONTAINING PROTEIN THAT CAN DRIVE THE MATURATION OF B-LYMPHOCYTES INTO IMMUNOGLOBULIN-SECRETING CELLS
BLIMP-1, A NOVEL ZINC FINGER-CONTAINING PROTEIN THAT CAN DRIVE THE MATURATION OF B-LYMPHOCYTES INTO IMMUNOGLOBULIN-SECRETING CELLS
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DOI：
10.1016/0092-8674(94)90321-2
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发表时间：
1994-04-22
期刊：
影响因子：
64.5
通讯作者：
DAVIS, MM
中科院分区：
文献类型：
--
作者：
TURNER, CA;MACK, DH;DAVIS, MM
我们描述了一种新的基因，Blimp-1（B淋巴细胞诱导的成熟蛋白），其转录本在B淋巴细胞分化为免疫球蛋白分泌细胞的过程中被快速诱导，并且其表达是晚期B和浆细胞系的特征。856个氨基酸的开放阅读框架包含5个Kruppel型锌指基序和与已知转录因子相似的富含脯氨酸的酸性区域。血清学研究表明，一个类似于100 kd的蛋白质，定位于细胞核。Blimp-1在B细胞淋巴瘤系中的稳定或瞬时转染导致与B细胞分化成早期浆细胞阶段相关的许多表型变化的表达，包括J链信息和免疫球蛋白分泌的诱导、Syndecan-1的上调以及细胞大小和粒度的增加。因此，Blimp-1似乎是一种多效性调节因子，能够至少部分地驱动B细胞的终末分化。
We describe a novel gene, Blimp-1 (for B lymphocyte-induced maturation protein), transcripts of which are rapidly induced during the differentiation of B lymphocytes into immunoglobulin secretory cells and whose expression is characteristic of late B and plasma cell lines. The 856 amino acid open reading frame contains five Kruppel-type zinc finger motifs and proline-rich and acidic regions similar to those of known transcription factors. Serological studies show an similar to 100 kd protein that localizes to the nucleus. Stable or transient transfection of Blimp-1 into B cell lymphoma lines leads to the expression of many of the phenotypic changes associated with B cell differentiation into an early plasma cell stage, including induction of J chain message and immunoglobulin secretion, up-regulation of Syndecan-1, and increased cell size and granularity. Thus, Blimp-1 appears to be a pleiotropic regulatory factor capable of at least partially driving the terminal differentiation of B cells.