Improvements to parallel plate flow chambers to reduce reagent and cellular requirements.
Improvements to parallel plate flow chambers to reduce reagent and cellular requirements.
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DOI：
10.1186/1471-2172-2-9
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发表时间：
2001
期刊：
影响因子：
3
通讯作者：
Larson RS
中科院分区：
文献类型：
--
作者：
Brown DC;Larson RS
平行板流动室已成为生理流动条件下白细胞检查的支柱。多年来发生了多次设计修改，但尚未对这些不同设计进行比较。此外，许多设计的试剂要求禁止对稀有白细胞群体的研究并且需要大量试剂。在本研究中，我们与 Lawrence 等人描述的原始平行板流动室相比，评估了对更新的平行板流动室设计的修改。我们表明，对腔室尺寸、内管直径、注射阀和再循环设计的修改可以在不改变测量的情况下显着减少细胞和试剂的需求。这些修改简单且易于实施，因此可以使用稀有或昂贵的试剂对稀有白细胞亚群进行研究。
The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur.