Acetylcholine receptor: evidence for a regulatory binding site in investigations of suberyldicholine-induced transmembrane ion flux in Electrophorus electricus membrane vesicles.
Acetylcholine receptor: evidence for a regulatory binding site in investigations of suberyldicholine-induced transmembrane ion flux in Electrophorus electricus membrane vesicles.
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乙酰胆碱受体：在电电泳膜囊泡中辛二胆碱诱导的跨膜离子通量研究中调节结合位点的证据。
DOI：
10.1021/bi00294a041
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Hess,GP
中科院分区：
文献类型：
--
作者：
Pasquale,EB;Takeyasu,K;Udgaonkar,JB;Cash,DJ;Severski,MC;Hess,GP
C. 使用时间分辨率为 5 ms 的淬灭流技术来测量放射性示踪离子 (86Rb+) 的跨膜通量。 JA（由受体活性形式介导的离子通量的速率系数）和a（离子通量失活的速率系数）随着辛二胆碱浓度的增加而增加，并在约15μ处达到稳定值。在较高的辛二胆碱浓度 (> 50 µ) 下，观察到离子通量速率随浓度而下降，而受体失活速率却没有相应的下降。乙酰胆碱或氨基甲酰胆碱没有观察到这种调节作用。先前提出的乙酰胆碱和氨基甲酰胆碱的最小动力学方案，通过包含辛二胆碱的额外调节配体结合位点进行修改，并以单一解离常数为特征，
C. A quench-flow technique with a time resolution of 5 ms was used to measure the transmembrane flux of a radioactive tracer ion (86Rb+). JA, the rate coefficient for ion flux mediated by the active formof the receptor, and a, the rate coefficient for the inactivation of the ion flux, increase with increasing suberyldicholine concentrations and reach a plateau value at about 15 µ. At higher suberyldicholine concentrations (> 50 µ), a concentration-dependent decrease in the ion flux rate was observed without a corresponding decrease in the rate of receptor inactivation. This regulatory effect was not observed with acetylcholine or carbamoylcholine. The minimal kinetic scheme previously presented for acetylcholine and carbamoylcholine, modified by the inclusion of an additional regulatory ligand-binding site for suberyldicholine and characterized by a single dissociation constant,