Protective effects of the selective alpha1A-adrenoceptor antagonist silodosin against cyclophosphamide-induced cystitis in rats
Protective effects of the selective alpha1A-adrenoceptor antagonist silodosin against cyclophosphamide-induced cystitis in rats
复制标题
选择性α1A肾上腺素受体拮抗剂西洛多辛对环磷酰胺所致大鼠膀胱炎的保护作用
DOI：
10.1016/j.jphs.2016.08.007
复制
发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Saito M
中科院分区：
文献类型：
--
作者：
Liu N;Shimizu S;Shimizu T;Nakamura K;Yamamoto M;Higashi Y;Saito M
我们观察了选择性α受体拮抗剂西洛多辛(Silod)对环磷酰胺(CyP)诱导的膀胱炎大鼠膀胱功能的保护作用，并对辣椒素敏感的传入神经通路进行了脱敏处理。雄性Wistar大鼠(310-400g)给予西洛德(0、100或300gμg/kg/d，P.O.)。两组均于测膀胱术前1周给予CYP(150 mg/kg，ip)。或实验前2天注射生理盐水。在另一项实验中，大鼠在膀胱测压前4天皮下注射CAP(125 mg/kg，S.C.)。对大鼠膀胱进行收获、称重和组织学评估。膀胱计量学评估显示，与赋形剂治疗的大鼠相比，CYP治疗的大鼠的收缩间期(ICI)、单次排尿量(SVV)和膀胱顺应性显著减少。大剂量西洛德或CAP治疗可显著增加CYP大鼠的ICI和SVV。然而，大剂量西洛德治疗并没有增加CAP处理的CYP大鼠的ICI和SVV。西洛德治疗不能改善CYP诱导的膀胱炎引起的膀胱重量、水肿和白细胞渗出。这些数据表明，西洛德阻断CAP1肾上腺素能受体可抑制α敏感的膀胱炎大鼠的传入通路。
We investigated the protective effects of a selective α1A-adrenoceptor antagonist, silodosin (Silod) on urinary bladder function in cyclophosphamide (CYP)-induced cystitis rats, with and without desensitization of the capsaicin (CAP)-sensitive afferent nerve pathway. Male Wistar rats (310–400 g) were pretreated with Silod (0, 100, or 300 μg/kg/day, p.o.) for 1 week before cystometry, and were administered either CYP (150 mg/kg, i.p.) or saline 2 days before the experiment. In another experiment, the rats were treated with CAP (125 mg/kg, s.c.) 4 days before the cystometry. The rat bladders were harvested, weighed, and evaluated histologically. The cystometric evaluation showed significant reductions in the intercontraction interval (ICI), single voided volume (SVV), and bladder compliance in CYP-treated rats compared to those in the vehicle-treated rats. High-dose Silod or CAP treatment significantly increased the ICI and SVV in the CYP rats. However, high-dose Silod treatment did not increase the ICI and SVV in CAP-treated CYP rats. Treatment with Silod did not improve the bladder weight, edema, and leukocyte infiltration resulting from the CYP-induced bladder inflammation. These data suggest that blockade of α1-adrenoceptors by Silod inhibited the CAP-sensitive afferent pathway in rats with cystitis.