Transcription Factor PecS Mediates Agrobacterium fabrum Fitness and Survival
Transcription Factor PecS Mediates Agrobacterium fabrum Fitness and Survival
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DOI：
10.1128/jb.00478-22
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发表时间：
2023-06-14
影响因子：
3.2
通讯作者：
Grove，Anne
中科院分区：
文献类型：
--
作者：
Nwokocha，George C.;Adhikari，Prava;Grove，Anne
转录调节因子 PecS 由选定的细菌病原体编码。例如，在植物病原体 Dickeya dadantii 中，PecS 控制一系列毒力基因，包括果胶酶基因和不同方向的基因pecM，该基因编码一个外排泵，通过该外排泵输出抗氧化剂靛蓝。在植物病原体法布农杆菌（以前称为根瘤农杆菌）中，pecS-pecM位点是保守的。使用A菌株。由于 fabrumin 的 pecS 已被破坏，我们在此表明 PecS 控制一系列与细菌适应性相关的表型。 PecS 抑制鞭毛运动和趋化性，这些过程对于 A 来说很重要。 fabrumto 到达植物伤口部位。在pecSdisruption菌株中，生物膜形成和微需氧存活率降低，而当pecSs破坏时，酰基高丝氨酸内酯（AHL）的产生和对活性氧（ROS）的抵抗力增加。 AHL 的产生和对 ROS 的抵抗预计与宿主环境特别相关。我们还表明 PecS 不参与处女基因的诱导。 PecS、尿酸盐和黄嘌呤的诱导配体可能存在于根际，并且它们在感染后在植物宿主内积累。因此，我们的数据表明 PecS 介导 A。重要的是，PecS 是一种在多种病原细菌中保守的转录因子，可调节毒力基因。植物病原体农杆菌的重要性不仅在于其在易感植物中诱导冠瘿，而且还在于其作为宿主植物遗传操作工具的作用。我们在这里表明A. fabrumPecS 控制一系列表型，这将使细菌在从根际过渡到宿主植物时具有优势。这包括信号分子的产生，这对于肿瘤诱导质粒的增殖至关重要。对感染过程的更全面的了解可能会为治疗感染以及促进顽固植物物种的转化提供信息。
The transcriptional regulator PecS is encoded by select bacterial pathogens. For instance, in the plant pathogen Dickeya dadantii, PecS controls a range of virulence genes, including pectinase genes and the divergently oriented genepecM, which encodes an efflux pump through which the antioxidant indigoidine is exported. In the plant pathogenAgrobacterium fabrum(formerly named Agrobacterium tumefaciens), thepecS-pecMlocus is conserved. Using a strain ofA. fabrumin whichpecShas been disrupted, we show here that PecS controls a range of phenotypes that are associated with bacterial fitness. PecS represses flagellar motility and chemotaxis, which are processes that are important forA. fabrumto reach plant wound sites. Biofilm formation and microaerobic survival are reduced in thepecSdisruption strain, whereas the production of acyl homoserine lactone (AHL) and resistance to reactive oxygen species (ROS) are increased whenpecSis disrupted. AHL production and resistance to ROS are expected to be particularly relevant in the host environment. We also show that PecS does not participate in the induction ofvirgenes. The inducing ligands for PecS, urate, and xanthine, may be found in the rhizosphere, and they accumulate within the plant host upon infection. Therefore, our data suggest that PecS mediatesA. fabrumfitness during its transition from the rhizosphere to the host plant.IMPORTANCEPecS is a transcription factor that is conserved in several pathogenic bacteria, where it regulates virulence genes. The plant pathogenAgrobacterium fabrumis important not only for its induction of crown galls in susceptible plants but also for its role as a tool in the genetic manipulation of host plants. We show here thatA. fabrumPecS controls a range of phenotypes, which would confer the bacteria an advantage while transitioning from the rhizosphere to the host plant. This includes the production of signaling molecules, which are critical for the propagation of the tumor-inducing plasmid. A more complete understanding of the infection process may inform approaches by which to treat infections as well as to facilitate the transformation of recalcitrant plant species.