Microbiological and molecular characterization of denitrification in biofilters treating pig manure
Microbiological and molecular characterization of denitrification in biofilters treating pig manure
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DOI：
10.1016/j.biortech.2007.06.066
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发表时间：
2008-07-01
影响因子：
11.4
通讯作者：
Lessard, Paul
中科院分区：
文献类型：
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作者：
Gilbert, Yan;Le Bihan, Yann;Lessard, Paul
曝气有机生物滤池处理猪粪具有一定的脱氮效果。为了优化这一过程，需要更好地理解其由细菌定殖。三个试点曝气生物过滤器，五天后与变量生物需氧量：总凯氏定氮（BOD5：TKN）的比率，喂养，建造和监测在180天。分析了系统中不同深度的气体和液体流中的氮气。通过评估生物过滤器内不同高度处的反硝化生物量的观察到的（氮质量平衡）和潜在的（适应的乙炔抑制技术）活性及其数量（nirS实时PCR）来表征反硝化生物量。反硝化作用被观察到，一旦硝酸盐产生的硝化菌，约40天的操作后，但潜在的反硝化增加从监测期开始。最高的BOD5：TKN比的生物过滤器与其他人表现出显着的差异，特别是在80天的操作后，因为它的潜在活性较低，观察到的硝酸盐去除率较高。数据表明，微生物主要集中在过滤器表面附近，从上到下存在微生物梯度。潜在的反硝化活性始终高于研究区域内观察到的活性，这表明生物质可以减少更多的硝酸盐，并且过滤器内发现的条件不允许反硝化完全发生。(c)2007爱思唯尔有限公司保留所有权利。
Aerated organic biofilters treating pig manure exhibit partial nitrogen removal. In order to optimize this process, a better comprehension of its colonization by denitrifiers was needed. Three pilot aerated biofilters, fed with variable Biological Oxygen Demand after five days: Total Kjeldahl Nitrogen (BOD5:TKN) ratios, were constructed and monitored during 180 days. Nitrogen was analyzed in the gaseous and liquid flows, at different depths in the systems. Denitrifying biomass was characterized by evaluating its observed (nitrogen mass balances) and potential (adapted acetylene inhibition technique) activities and its quantity (real-time PCR on nirS), at different heights inside the biofilters. Denitrification was observed as soon as nitrate was produced by nitrifiers, after approximately 40 days of operation, but the potential to denitrify increased from the beginning of the monitoring period. Biofilter fed with the highest BOD5:TKN ratio showed significant differences with the others, particularly after 80 days of operation, as its potential activity was lower with a higher observed nitrate removal. Data showed that denitrifiers were mainly localized near the surface of the filter and that a microbiological gradient was present from top to bottom. The potential denitrifying activities were always higher than what was being observed inside the sections studied, suggesting that the biomass could have reduced more nitrate and that conditions found inside the filter did not allow denitrification to completely occur. (c) 2007 Elsevier Ltd. All rights reserved.