Nano Au@Cu2-xS with near-infrared photothermal and peroxidase catalytic activities redefines efficient antibiofilm-oriented root canal therapy
Nano Au@Cu2-xS with near-infrared photothermal and peroxidase catalytic activities redefines efficient antibiofilm-oriented root canal therapy
复制标题
具有近红外光热和过氧化物酶催化活性的纳米Au@Cu2-xS重新定义了高效的抗菌膜导向根管治疗
DOI：
10.1016/j.cej.2021.130090
复制
发表时间：
2021-05-04
影响因子：
15.1
通讯作者：
Hu, Ji-Ming
中科院分区：
文献类型：
--
作者：
Cao, Jun;Sun, Qing;Hu, Ji-Ming
杀死生物膜中的有害细菌是当前根管治疗的主要挑战。传统的化学消毒剂虽然对浮游生物具有良好的杀菌效果，但对于细菌生物膜的去除和灭菌效果甚微，甚至导致细菌产生耐药性和较高的毒副作用。在此，我们首次提出生物安全纳米复合材料，该复合材料集成了光热和过氧化物酶样催化活性，可有效去除和杀死根管中的细菌生物膜。金（核）和硫化铜（I、II）（壳）的结合表现出优异的催化效果，可以催化生物安全浓度的过氧化氢分解为羟基自由基，从而有效去除生物膜。此外，对抗生物膜机理的研究表明，纳米复合材料（Au@Cu2-xS）对生物膜的主要成分蛋白质和多糖具有显着的降解作用。体外结果表明，与单独自由基和热处理相比，自由基和热处理的协同作用对革兰氏阳性粪肠球菌和革兰氏阴性梭杆菌核具有更高的杀菌率。更重要的是，对孤立牙片、根管模型和比格犬的杀菌结果表明，Au@Cu2-xS 对灭活生物膜中的细菌非常有效。因此，这种安全且协同的抗菌系统在以抗菌膜为导向的根管治疗中具有巨大的潜力。
Killing harmful bacteria in biofilms is a major challenge for current root canal therapy. Although traditional chemical disinfectants have a good bactericidal effect on plankton, they have little efficiency for the removal and sterilization of bacterial biofilms, and even lead to the emergence of bacterial resistance and high toxic side effects. Herein, we propose for the first time biosafe nanocomposites that integrate photothermal and peroxidaselike catalytic activity for efficient removal and killing of bacterial biofilms in root canals. The integration of gold (core) and copper (I, II) sulfide (shell) shows excellent catalytic effect, which can catalyze the decomposition of hydrogen peroxide at biosafe concentrations to hydroxyl radicals for efficient removal of biofilms. Moreover, studies on the mechanism of antibiofilm showed that the nanocomposites (Au@Cu2-xS) have significant degradation effects on proteins and polysaccharides-the main components of biofilm. The in vitro results indicated that the synergism of free radical and heat shows higher sterilization rate against both gram-positive Enterococcus faecalis and gram-negative Fusobacterium nucleus, compared with free radical and heat treatment alone. More importantly, the bactericidal results on isolated dental slices and in root canal models and beagle dog demonstrated the Au@Cu2-xS is highly effective in inactivating the bacteria in biofilms. Hence this safe and synergistic antimicrobial system has great potential in antibiofilm-oriented root canal therapy.