DNA microarray technology in toxicogenomics of aquatic models: Methods and applications
DNA microarray technology in toxicogenomics of aquatic models: Methods and applications
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DOI：
10.1016/j.cbpc.2006.04.017
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Walter, Ronald B.
中科院分区：
文献类型：
--
作者：
Ju, Zhenlin;Wells, Melissa C.;Walter, Ronald B.
毒理基因组学代表毒理学与基因组学和生物信息学的融合，以研究基因组响应环境污染物的生物学功能。水生物种传统上被用作毒理学模型来表征环境胁迫的作用。最近完成的几种鱼类 DNA 测序刺激了 DNA 微阵列的发展，使研究人员能够采用毒理学方法。然而，由于微阵列技术迄今为止仅限于少数水生物种，并且从微阵列数据中推导出生物学意义高度依赖于统计论证，因此微阵列在水生物种研究中的全部潜力尚未实现。在此，我们回顾了与 DNA 微阵列的构建、探针设计、统计和生物信息数据分析以及当前应用相关的一些问题。作为一个模型，最近开发的 rnedaka (Oryzias latipes) 寡核苷酸微阵列被描述为强调与阵列技术及其在暴露于缺氧的水生物种中的应用相关的一些问题。尽管微阵列数据中存在已知的非生物变异，但毫无疑问阵列技术将对水生毒理学产生巨大影响。微阵列在水生毒物基因组学中的应用范围包括从诊断生物标志物的发现到建立应激特异性特征和标志着适应新环境条件的分子途径。 (c) 2006 Elsevier Inc. 保留所有权利。
Toxicogenomics represents the merging of toxicology with genomics and bioinfoarmatics to investigate biological functions of genome in response to environmental contaminants. Aquatic species have traditionally been used as models in toxicology to characterize the actions of environmental stresses. Recent completion of the DNA sequencing for several fish species has spurred the development of DNA microarrays allowing investigators access to toxicogenomic approaches. However, since microarray technology is thus far limited to only a few aquatic species and derivation of biological meaning from microarray data is highly dependent on statistical arguments, the full potential of microarray in aquatic species research has yet to be realized. Herein we review some of the issues related to construction, probe design, statistical and bioinformatical data analyses, and current applications of DNA microarrays. As a model a recently developed rnedaka (Oryzias latipes) oligonucleotide microarray was described to highlight some of the issues related to array technology and its application in aquatic species exposed to hypoxia. Although there are known non-biological variations present in microarray data, it remains unquestionable that array technology will have a great impact on aquatic toxicology. Microarray applications in aquatic toxicogenomics will range from the discovery of diagnostic biomarkers, to establishment of stress-specific signatures and molecular pathways hallmarking the adaptation to new environmental conditions. (c) 2006 Elsevier Inc. All rights reserved.