COMPARISON OF FORMALIN-ETHYL ETHER SEDIMENTATION, FORMALIN-ETHYL ACETATE SEDIMENTATION, AND ZINC-SULFATE FLOTATION TECHNIQUES FOR DETECTION OF INTESTINAL PARASITES

COMPARISON OF FORMALIN-ETHYL ETHER SEDIMENTATION, FORMALIN-ETHYL ACETATE SEDIMENTATION, AND ZINC-SULFATE FLOTATION TECHNIQUES FOR DETECTION OF INTESTINAL PARASITES
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DOI:
10.1128/jcm.13.5.882-884.1981
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发表时间:
1981-01-01
影响因子:
9.4
通讯作者:
SMITH, JH
SMITH, JH
中科院分区:
医学2区
文献类型:
--
作者:
TRUANT, AL;ELLIOTT, SH;SMITH, JH

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使用 250 多个临床寄生虫学标本对福尔马林-乙醚沉降、福尔马林-乙酸乙酯沉降和硫酸锌浮选技术进行了比较。鉴定出 50 个阳性样本,并发现了多种寄生虫,包括变形虫、鞭毛虫、绦虫、线虫和吸虫。福尔马林-乙醚和福尔马林-乙酸乙酯沉降程序在检测包囊、卵和幼虫方面给出了相同的结果,并且这些方法在检测选定的卵方面比浮选程序具有优势。硫酸锌方法对于检测原生动物包囊、膜壳虫和钩虫卵更有效。福尔马林-乙酸乙酯程序显然提供了福尔马林-乙醚法的合适替代方法,并且证明了在粪便标本寄生虫分析中使用浮选和沉降程序的价值。
Formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes and trematodes, were encountered. The formalin-ether and formalin-ethyl acetate sedimentation procedures gave identical results for the detection of cysts, ova, and larvae, and these methods offered an advantage over the flotation procedure for the detection of selected ova. The zinc sulfate procedure was more effective for the detection of protozoan cysts, Hymenolepis nana, and hookworm eggs. The Formalin-ethyl acetate procedure evidently provides a suitable alternative to the formalin-ether method, and they demonstrate the value of using both flotation and sedimentation procedure in the analysis of fecal specimens for parasites.