Prevention of postoperative adhesion with a colloidal gel based on decyl group-modified Alaska pollock gelatin microparticles
Prevention of postoperative adhesion with a colloidal gel based on decyl group-modified Alaska pollock gelatin microparticles
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基于癸基改性阿拉斯加明胶微粒的胶体凝胶预防术后粘连
DOI：
10.1016/j.actbio.2022.06.014
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发表时间：
2022
影响因子：
9.7
通讯作者：
Taguchi Tetsushi
中科院分区：
文献类型：
--
作者：
Ito Shima;Nishiguchi Akihiro;Ichimaru Hiroaki;Nagasaka Kazuhiro;Hirade Hiromi;Taguchi Tetsushi
术后粘连，即腹壁与受损器官的粘连，在腹部手术后会引起严重的并发症。尽管存在物理屏障（即溶液、薄膜和水凝胶），但在湿组织中具有稳定性和易于使用的单物质系统的防粘材料尚未被报道。在这里，我们报告了一种基于微粒的，可喷涂的防粘材料，包括癸基改性阿拉斯加鳕鱼明胶（C10-ApGltn）。采用凝聚法、冷冻干燥法和热交联法制备了C10-ApGltn微粒子（C10-MPs）。在不使用交联剂的情况下，C10-MPs通过水合作用在肠浆膜组织上粘附形成胶凝胶层。C10-MPs水化后，形成的胶体凝胶层不粘附于其他组织。胃浆膜组织上形成的C10-MP胶凝胶层在生理盐水中浸泡2天后表现出稳定性。胶凝胶层也表现出组织随动性。动物蛋白黏附模型显示，盲肠和腹壁缺损上的C10-MP胶凝胶层可有效降低术后黏附，诱导组织重塑，包括再间皮化。因此，C10-MPs是防止术后粘连的潜在抗粘连材料。我们评估了一种基于癸基改性阿拉斯加鳕鱼明胶（ApGltn）微粒（C10-MPs）的胶体凝胶的术后粘连预防能力。这些微粒是可喷洒的，在胃肠道组织上形成胶状凝胶，仅水合作用。我们在体外实验中发现，将癸基修饰成ApGltn通过疏水相互作用提高了C10-MP胶体凝胶在组织上的稳定性。凝胶通过在体内黏附模型中作为物理屏障来防止术后黏附。
Postoperative adhesion, bonding of the abdominal wall to damaged organs, causes severe complications after abdominal surgery. Despite the availability of physical barriers (i.e., solutions, films, and hydrogels), adhesion prevention materials that are a single-substance system with stability in wet tissue and ease of use have not been reported. Here, we report a microparticle based, sprayable adhesion prevention material comprising decyl group modified Alaska pollock gelatin (C10-ApGltn). C10-ApGltn microparticles (C10-MPs) were prepared by a coacervation method, freeze drying, and thermal crosslinking. The C10-MPs adhered to and formed a colloidal gel layer on intestinal serosal tissue by hydration without any crosslinking agents. After hydration of the C10-MPs, the resulting colloidal gel layer did not adhere to other tissues. Additionally, the C10-MP colloidal gel layer formed on the stomach serosal tissue showed stability when submersed in saline for 2 days. The colloidal gel layer also showed tissue followability. Anin vivorat adhesion model revealed that C10-MP colloidal gel layer on the cecum and abdominal wall defects effectively reduced postoperative adhesion and induced tissue remodeling, including re-mesothelialization. Therefore, C10-MPs are a potential anti-adhesion material for preventing postoperative adhesion.Statement of significanceWe evaluated the postoperative adhesion prevention ability of a colloidal gel based on decyl group modified Alaska pollock gelatin (ApGltn) microparticles (C10-MPs). These microparticles are sprayable and form a colloidal gel with only hydration on the gastrointestinal tissue. We revealed that the modification of the decyl group into ApGltn improved the stability of C10-MP colloidal gel on the tissue by hydrophobic interaction in thein-vitroexperiments. The gel prevented postoperative adhesion by being a physical barrier in thein-vivorat adhesion model.