Possible involvement of the expression and phosphorylation of N-Myc in the induction of HMGA1a by hypoxia in the human neuroblastoma cell line
Possible involvement of the expression and phosphorylation of N-Myc in the induction of HMGA1a by hypoxia in the human neuroblastoma cell line
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DOI：
10.1016/j.neulet.2004.10.039
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发表时间：
2005-02
影响因子：
2.5
通讯作者：
Takeshi Yanagita;T. Manabe;H. Okuda;S. Matsuzaki;Y. Bando;T. Katayama;M. Tohyama
中科院分区：
文献类型：
--
作者：
Takeshi Yanagita;T. Manabe;H. Okuda;S. Matsuzaki;Y. Bando;T. Katayama;M. Tohyama
缺氧后SK-N-SH细胞核内N-Myc和高迁移率族蛋白A1 a（HMGA 1a）表达增加。这些观察结果伴随着额外的高分子量条带的出现，这些条带通过碱性磷酸酶预处理消除。免疫沉淀显示，磷酸化的丝氨酸，苏氨酸和酪氨酸残基的N-Myc的细胞核。这些结果表明，缺氧诱导的信号在SK-N-SH细胞中导致HMGA 1a的持续表达，这可能诱导转录因子N-Myc的表达，并且N-Myc的丝氨酸、苏氨酸和酪氨酸残基的磷酸化在刺激后的早期阶段发生。这种信号巩固过程可能在神经元缺氧后的神经元存活中发挥作用。
Increased expression of N-Myc and expression of the high mobility group protein A1a (HMGA1a) were observed in the nuclei of SK-N-SH cells following exposure to hypoxia. These observations were accompanied by the appearance of additional high molecular weight bands, which were eliminated by pretreatment with alkaline phosphatase. Immunoprecipitation showed phosphorylation of serine, threonine and tyrosine residues of N-Myc in the nucleus. These results suggest that hypoxia-induced signals in SK-N-SH cells lead to persistent expression of HMGA1a, which may induce expression of the transcription factor N-Myc, and that phosphorylation at serine, threonine and tyrosine residues of N-Myc occurs at an early stage after stimulation. Such signal consolidation processes could play a role in neuronal survival after hypoxia in neurons.