Role of histone tails in the conformation and interactions of nucleosome core particles
Role of histone tails in the conformation and interactions of nucleosome core particles
复制标题
DOI：
10.1021/bi036210g
复制
发表时间：
2004-04-27
期刊：
影响因子：
2.9
通讯作者：
Livolant, F
中科院分区：
文献类型：
--
作者：
Bertin, A;Leforestier, A;Livolant, F
这项工作的目的是测试组蛋白尾部在高于临界盐浓度的核小体之间出现吸引相互作用中的作用，该临界盐浓度对应于核小体外部的完整尾部延伸[Mangenot, S., et al (2002) Biophys. J.82, 345-356; Mangenot, S. 等人 (2002) 欧洲。物理。 J.E 7, 221-231]。小角度X射线散射实验与DNA的146::L 3 完整核小体和胰蛋白酶尾部缺失的核小体平行进行。我们将一价盐浓度从 10 变化到 300 一价盐浓度，并跟踪 (i) 表征颗粒之间相互作用的第二维里系数和 (ii) 颗粒构象的演变。在没有尾巴的情况下，有吸引力的相互作用不会出现，这验证了所提出的假设。
The goal of this work was to test the role of the histone tails in the emergence of attractive interactions between nucleosomes above a critical salt concentration that corresponds to the complete tail extension outside the nucleosome [Mangenot, S., et al (2002) Biophys. J. 82, 345-356; Mangenot, S., et al (2002) Eur. Phys. J. E 7, 221-231]. Small angle X-ray scattering experiments were performed in parallel with intact and trypsin tail-deleted nucleosomes with 146 ::L 3 by DNA. We varied the monovalent salt concentration from 10 to 300 monovalent salt concentration and followed the evolution of (i) the second virial coefficient that characterizes the interactions between particles and (ii) the conformation of the particle. The attractive interactions do not emerge in the absence of the tails, which validates the proposed hypothesis.