Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks
Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks
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DOI：
10.1139/cjm-47-3-229
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Azevedo, JL
中科院分区：
文献类型：
--
作者：
Araújo, WL;Maccheroni, W;Azevedo, JL
从几个柑橘砧木表面消毒的叶片组织中分离到真菌和细菌。分离到的主要菌种为产碱杆菌、芽孢杆菌。(包括蜡状杆菌、香菇、巨型杆菌、短小芽孢杆菌和枯草杆菌)、洋葱伯克霍尔德氏菌、枯草杆菌、阴沟肠杆菌、甲基杆菌、聚集性巴氏杆菌，其中聚集性伯克霍尔德氏菌和短小芽孢杆菌是分离频率最高的菌种。真菌种类最丰富的是炭疽菌、柑橘炭疽菌和枝孢霉。利用随机扩增多态DNA技术对36株内生细菌的遗传多样性进行了分析，结果表明短小芽孢杆菌的遗传多样性高于聚集性芽孢杆菌，但遗传多样性与寄主植物无关。柠檬酸杆菌与几种常见内生细菌的体外相互作用研究表明，柠檬酸杆菌分泌的代谢产物对某些芽孢杆菌有抑制作用，对聚集性芽孢杆菌有促进生长作用。
Fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. The principal bacterial species isolated were Alcaligenes sp., Bacillus spp. (including B. cereus, B. lentus, B. megaterium, B, pumilus, and B. subtilis), Burkholderia cepacia, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium extorquens, and Pantoea agglomerans, with P. agglomerans and B. pumilus being the most frequently isolated species. The most abundant fungal species were Colletotrichum gloeosporioides, Guignardia citricarpa, and Cladosporium sp. Genetic variability between 36 endophytic bacterial isolates was analysed by the random amplified polymorphic DNA (RAPD) technique, which indicated that B. pumilus isolates were more diverse than P. agglomerans isolates, although genetic diversity was not related to the host plants. In vitro interaction studies between G. citricarpa isolates and the most frequently isolated endophytic bacteria showed that metabolites secreted by G. citricarpa have an inhibitory growth effect on some Bacillus species, and a stimulatory growth effect on P. agglomerans.