Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt)
Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt)
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DOI：
10.1007/s10529-010-0392-8
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发表时间：
2010
影响因子：
2.7
通讯作者：
Chengjian Xie;Beibei Zhang;De Wang;Fei Kou;Xupeng Zhao;Xingyong Yang
中科院分区：
文献类型：
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作者：
Chengjian Xie;Beibei Zhang;De Wang;Fei Kou;Xupeng Zhao;Xingyong Yang
LJAMP 1是一种小的抗菌蛋白，以前从益母草种子中纯化，它优先在种子中表达。利用基因组步移法分离了ATG起始密码子上游794 bp的序列，并将其克隆到GUS报告基因的上游，以确定GUS的组织特异性表达模式。转基因烟草表明，pLJAMP 1启动子衍生的GUS报告基因在花粉、瘦果和种子中特异表达。顺式作用元件分析还表明pLJAMP 1启动子含有与花粉和种子相关的转录调控元件。
LJAMP1 is a small antimicrobial protein purified previously from the seeds of motherwort, and it is expressed preferentially in seeds. A 794-bp upstream sequence of the ATG start codon was isolated using a genome walking method and cloned into the upstream of the β-glucuronidase (GUS) reporter gene to determine the GUS tissue-specific expression pattern. The transgenic tobacco showed thatpLJAMP1promoter derived GUS reporter gene special expression in pollen, achene and seed. The analysis ofcis-acting elements also revealedpLJAMP1promoter contained pollen and seed related transcriptional control elements.