Truncated and RIP-degenerated copies of the LTR retrotransposon Pholy are clustered in a pericentrorneric region of the Leptosphaeria maculans genome
Truncated and RIP-degenerated copies of the LTR retrotransposon Pholy are clustered in a pericentrorneric region of the Leptosphaeria maculans genome
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DOI：
10.1016/j.fgb.2004.09.004
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发表时间：
2005-01-01
影响因子：
3
通讯作者：
Rouxel, T
中科院分区：
文献类型：
--
作者：
Attard, A;Gout, L;Rouxel, T
Taylor和Borgmann [Mol. Plant microinteraction . 7(1994) 181]将maculans钩端螺旋体（Leptosphaeria maculans）的LMR1 5.2 kb分散重复序列描述为与LINEs和sin具有同源性的未知重复元件。本研究以LMR1序列为模板，对分离物v23.1.3的2.80 Mb染色体的近中心点区域对应的184 kb基因组序列进行了全长元件鉴定。该区域包括(i)一个6980 bp的全尺寸Pholy元件，由两个275- 280 bp的长末端重复序列（lts）包围，（ii）五个Pholy相关序列，通常在它们的3'端截断，以及（iii）五个单独的lts。结构特征强烈表明，Pholy对应于一个古代复制样反转录转座子，与Stagonospora nodorum的Elsa反转录转座子具有很强的同源性。也有人认为这种现象是中心点周围区域特有的。对184kb序列和基因组其他部分的pholy样序列结构的比较分析表明，该重复序列家族在广泛重复诱导点突变（RIP）后高度退化。(C) 2004爱思唯尔公司版权所有。
The LMR1 5.2 kb interspersed repeat of Leptosphaeria maculans was described by Taylor and Borgmann [Mol. Plant Microbe Interact. 7 (1994) 181] as an uncharacterized repeated element sharing homologies with both LINEs and SINEs. Here, we used the LMR1 sequence as a template to identify the full-length element within a 184-kb genomic sequence corresponding to the pericentromeric region of the 2.80 Mb chromosome of isolate v23.1.3. This region comprises (i) one 6980-bp full-sized Pholy element bordered by two 275- to 280-bp long terminal repeats (LTRs), (ii) five Pholy-related sequences, usually truncated at their 3' ends, and (iii) five solo-LTRs. Structural features strongly suggested that Pholy corresponds to an ancient copia-like retrotransposon, sharing strong homologies with the Elsa retrotransposon of Stagonospora nodorum. Pholy was also suggested to be specific to pericentromeric regions. Comparative analysis of the structure of the Pholy-like sequences occurring in the 184-kb contig and in other parts of the genome showed that this family of repeats is highly degenerated following extensive repeat induced point mutation (RIP). (C) 2004 Elsevier Inc. All rights reserved.