Development of surface adhesion in Caulobacter crescentus
Development of surface adhesion in Caulobacter crescentus
复制标题
DOI：
10.1128/jb.186.5.1438-1447.2004
复制
发表时间：
2004-03-01
影响因子：
3.2
通讯作者：
Brun, YV
中科院分区：
文献类型：
--
作者：
Bodenmiller, D;Toh, E;Brun, YV
新月形茎杆菌的生命周期由活动期和无根期组成。在无柄阶段，月牙草经常被发现通过它的黏附物紧密地附着在表面上。在这项研究中，我们研究了生长和外部结构对月牙草附着在非生物表面的贡献。我们表明，保持是必要的，但不是充分的最佳连接。相反，月牙草的粘附是一个复杂的发育过程。我们发现月牙草附着于表面是受细胞周期调节的，生长或能量或两者都是这一过程所必需的。附着的初始阶段发生在群细胞中，并由鞭毛运动和毛促进。我们的研究结果表明，当群细胞分化为有柄细胞时，强附着是由一个固定物的合成介导的。
Caulobacter crescentus has a dimorphic life cycle composed of a motile stage and a sessile stage. In the sessile stage, C. crescentus is often found tightly attached to a surface through its adhesive holdfast. In this study, we examined the contribution of growth and external structures to the attachment of C. crescentus to abiotic surfaces. We show that the holdfast is essential but not sufficient for optimal attachment. Rather, adhesion in C. crescentus is a complex developmental process. We found that the attachment of C. crescentus to surfaces is cell cycle regulated and that growth or energy or both are essential for this process. The initial stage of attachment occurs in swarmer cells and is facilitated by flagellar motility and pili. Our results suggest that strong attachment is mediated by the synthesis of a holdfast as the swarmer cell differentiates into a stalked cell.