Cellulomonas composti sp nov., a cellulolytic bacterium isolated from cattle farm compost
Cellulomonas composti sp nov., a cellulolytic bacterium isolated from cattle farm compost
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DOI：
10.1099/ijs.0.63974-0
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发表时间：
2007-06-01
影响因子：
2.8
通讯作者：
Lee, Sung-Taik
中科院分区：
文献类型：
--
作者：
Kang, Myung-Suk;Im, Wan-Taek;Lee, Sung-Taik
从韩国大田附近的一个养牛场的堆肥中分离到一株具有纤维素酶和β-葡萄糖苷酶活性的细菌菌株TR 706（T）。这是一种革兰氏阳性、需氧或兼性厌氧、非运动、杆状细菌。基于16 S rRNA基因序列的系统发育分析表明，该菌株属于纤维单胞菌属（CellulomonasudaDSM 20107（T）），与GellulomonasudaDSM 20107（T）的同源性最高（98.5%）。细胞壁分析显示存在A4 β型L-Orn-D-Glu肽聚糖。检测到的细胞壁糖是甘露糖和葡萄糖。主要的甲基萘醌是MK-9（H-4）;检测到的MK-8（H-4）数量较少。主要脂肪酸为antiso-C-15：0、C-16：0、C-14：0和C-18：0。检测到的极性脂质为二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇。DNA-DNA杂交和生理生化试验结果表明，TR 7 -06（T）代表一个新种。结合基因型和表型数据表明菌株TR 706（T）（= KCTC 19030（T）=NBRC 100758（T））值得描述为一种新的纤维单胞菌属物种，堆肥纤维单胞菌新种的模式菌株。
A bacterial strain, TR706(T), which has cellulase and beta-glucosiclase activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. It was a Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas, with highest sequence similarity to Gellulomonas uda DSM 20107(T) (98.5 %). Cell wall analysis revealed the presence of type A4 beta, L-Orn-D-Glu pepticloglycan. The cell-wall sugars detected were mannose and glucose. The predominant menaquinone was MK-9(H-4); MK-8(H-4) was detected in smaller quantities. The major fatty acids were anteiso-C-15:0, C-16:0, C-14:0 and C-18:0. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that TR7-06(T) represents a novel species. The combined genotypic and phenotypic data show that strain TR706(T) (= KCTC 19030(T) =NBRC 100758(T)) merits description as the type strain of a novel Cellulomonas species, Cellulomonas composti sp. nov.