ANTIGENS OF TYPE-1 FIMBRIAE OF SALMONELLAE AND OTHER ENTEROBACTERIA
ANTIGENS OF TYPE-1 FIMBRIAE OF SALMONELLAE AND OTHER ENTEROBACTERIA
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DOI：
10.1099/00222615-2-4-535
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发表时间：
1969-01-01
影响因子：
3
通讯作者：
CAMPBELL, I
中科院分区：
文献类型：
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作者：
DUGUID, JP;CAMPBELL, I
确定凝集反应的抗原，并从O-，H-和VI-抗原不同，被证明在1型菌毛的细菌在沙门氏菌的菌毛相文化。当生长足够长的时间时，大多数沙门氏菌菌株产生流苏状培养物，例如，24-48小时，在37°C有氧静态肉汤中。当在肉汤中仅生长6小时、在葡萄糖肉汤中生长12小时或在琼脂平板上生长24小时时，相同的菌株产生缺乏菌毛抗原的非菌毛期培养物。菌毛抗原不依赖于O-、H-和Vi-抗原，这一发现表明，抗菌毛期培养物的抗血清通过非菌毛期细菌的吸收而不含O-、H-和其他非菌毛凝集素（“纯菌毛抗血清”）强凝集的菌毛相细菌，但不凝集非菌毛期细菌或已通过在100°C下加热而被脱纤化的菌毛细菌。几种沙门氏菌血清型菌株的（未吸收的）和纯的（吸收的）菌毛抗血清凝集多种异源血清型的菌毛相细菌。反应表明，在79个血清型的沙门氏菌和亚利桑那和柠檬酸杆菌各2个菌株的95个菌毛菌株中存在共同的菌毛抗原。吸收结果表明，除了某些血清型菌株中常见的菌毛抗原外，还存在其他菌毛抗原，但相同的抗原存在于相同血清型的不同菌株中。在福氏志贺菌、大肠杆菌和产气克雷伯菌中发现了一种共同的菌毛抗原，但这组细菌与沙门氏菌-亚利桑那-柠檬酸杆菌组之间没有共同的抗原。除非仅使用非菌毛细菌制备凝集悬液和生产诊断抗血清，否则在诊断凝集试验中可能获得误导性的交叉反应。在已感染沙门氏菌或用含有菌毛抗原的TAB疫苗免疫的人中，交叉反应的菌毛凝集素的发展可能导致肥达试验中获得假阳性反应。
Antigens that determine agglutination reactions, and are distinct from the O-, H- and Vi-antigens, were demonstrated in the type-1 fimbriae of bacteria in fimbriate-phase cultures of salmonellae. Most strains of salmonellae produced fimbriate cultures when grown for a sufficient period, e.g., 24–48 hr, at 37°C in aerobic static broth. The same strains produced non-fimbriate-phase cultures lacking fimbrial antigens when grown for only 6 hr in broth, for 12 hr in glucose broth or for 24 hr on an agar plate. The independence of the fimbrial antigens from the O-, H- and Vi-antigens was shown by the finding that an antiserum raised against a fimbriate-phase culture and freed from O-, H- and other non-fimbrial agglutinins by absorption with non-fimbriate-phase bacteria (“pure fimbrial antiserum”) strongly agglutinated fimbriate-phase bacteria, but did not agglutinate either non-fimbriate-phase bacteria or fimbriate bacteria that had been defimbriated by heating at 100°C.Crude (unabsorbed) and pure (absorbed) fimbrial antisera to strains of several salmonella serotypes agglutinated fimbriate-phase bacteria of a wide variety of heterologous serotypes. The reactions indicated the presence of a common fimbrial antigen in all of 95 fimbriate strains in 79 serotypes ofSalmonellaand two strains each ofArizonaandCitrobacter. Absorption findings suggested that other fimbrial antigens were present in addition to the common one in strains of certain serotypes, but that the same antigens were present in different strains of the same serotype. A common fimbrial antigen was demonstrated in strains ofShigella flexneri, Escherichia coliandKlebsiella aerogenes, but there was no sharing of antigens between this group of bacteria and the salmonella-arizona-citrobacter group.Misleading cross-reactions may be obtained in diagnostic agglutination tests unless only non-fimbriate bacteria are used for the preparation of agglutinable suspensions and the production of diagnostic antisera. The development of cross-reacting fimbrial agglutinins in persons who have been infected with a salmonella or immunised with a TAB vaccine containing fimbrial antigens may lead to false-positive reactions being obtained in Widal tests.