Prediction by 31P‐NMR of the irreversibility of ischemic injury in rat skeletal muscle after ligation of the femoral artery
Prediction by 31P‐NMR of the irreversibility of ischemic injury in rat skeletal muscle after ligation of the femoral artery
复制标题
31P-NMR预测股动脉结扎后大鼠骨骼肌缺血性损伤的不可逆性
DOI：
10.1002/mus.880120105
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发表时间：
1989
期刊：
影响因子：
3.4
通讯作者：
M. Décorps
中科院分区：
文献类型：
--
作者：
B. Authier;A. Rossi;J. Albrand;M. Décorps
1小时前通过股动脉结扎使大鼠腿部肌肉缺血，然后用电刺激坐骨神经进行20分钟的运动。31P - NMR波谱用于监测运动前、运动中和运动后肌肉中高能磷酸盐含量的变化。研究的35块肌肉中有15块逐渐演变为完全坏死，而其他肌肉在运动后2-5小时内显示出恢复的迹象。那些随后没有恢复的肌肉在休息时（运动前）比恢复的肌肉含有更多的无机磷酸盐（Pi）。提示急性缺血时静息Pi水平与血流受限程度相关，可用于预测缺血的严重程度。
Rat leg muscles, rendered ischaemic 1 hour previously by ligation of the femoral artery, were submitted to 20 minutes exercise by electrical stimulation of the sciatic nerve. 31P‐NMR spectroscopy was used to monitor the changes in high‐energy phosphate content of the muscles before, during, and after exercise. Fifteen of the 35 studied muscles evolved toward total necrosis, whereas the others showed signs of recovery over a 2–5‐hour postexercise period. Those muscles which did not subsequently recover contained significantly more inorganic phosphate (Pi) at rest (before exercise) than those which recovered. It is suggested that under acute ischaemic conditions the Pi level at rest is correlated with the extent of blood flow restriction and can be used to predict the severity of the ischemia.