Adult-like complexity of the larval antennal lobe of D-melanogaster despite markedly low numbers of odorant receptor neurons
Adult-like complexity of the larval antennal lobe of D-melanogaster despite markedly low numbers of odorant receptor neurons
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DOI：
10.1002/cne.10188
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发表时间：
2002-04-15
影响因子：
2.5
通讯作者：
Stocker, RF
中科院分区：
文献类型：
--
作者：
Python, F;Stocker, RF
我们提供了一个详细的分析果蝇幼虫头部的化学感受系统，基于共聚焦显微镜的细胞特异性报告基因表达的P[GAL 4]增强子陷阱线。特别是，我们描述了三个外部和三个咽化学感觉器官的神经元组成，神经束选择其传入，和他们的中央目标区域。由于总共有21个嗅觉神经元和80个味觉神经元，感觉水平在数字上比成人简单得多。此外，它的设计与成人不同，表现出嗅觉和味觉感受器之间的联系。与此相反，一阶中继的嗅觉传入，幼虫触角叶（LAL），表现出成人一样的功能，无论是在结构和细胞数量。它显示了一个分裂成大约30个亚单位，让人想起在成年触角叶肾小球。两者合计，幼虫的化学感受系统的设计是一个“混合体”，与幼虫的具体特点，在外围和中央的特点，共同与成人。传入神经的数量大大减少，并且LAL和成年触角叶的结构相似，使得果蝇的幼虫化学感觉系统成为一个有价值的模型系统，用于研究嗅觉和味觉以及研究其成年组织的发展。(C)2002 Wiley-Liss，Inc.
We provide a detailed analysis of the larval head chemosensory system of Drosophila melanogaster, based on confocal microscopy of cell-specific reporter gene expression in P[GAL4] enhancer trap lines. In particular, we describe the neuronal composition of three external and three pharyngeal chemosensory organs, the nerve tracts chosen by their afferents, and their central target regions. With a total of 21 olfactory and 80 gustatory neurons, the sensory level is numerically much simpler than that of the adult. Moreover, its design is different than in the adult, showing an association between smell and taste sensilla. In contrast, the first-order relay of the olfactory afferents, the larval antennal lobe (LAL), exhibits adult-like features both in terms of structure and cell number. It shows a division into approximately 30 subunits, reminiscent of glomeruli in the adult antennal lobe. Taken together, the design of the larval chemosensory system is a "hybrid," with larval-specific features in the periphery and central characteristics in common with the adult. The largely reduced numbers of afferents and the similar architecture of the LAL and the adult antennal lobe, render the larval chemosensory system of Drosophila a valuable model system, both for studying smell and taste and for examining the development of its adult organization. (C) 2002 Wiley-Liss, Inc.