Differences in embryo quality are associated with differences in oocyte composition: A proteomic study in inbred mice
Differences in embryo quality are associated with differences in oocyte composition: A proteomic study in inbred mice
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DOI：
10.1002/pmic.201400334
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发表时间：
2015-02-01
期刊：
影响因子：
3.4
通讯作者：
Boiani, Michele
中科院分区：
文献类型：
--
作者：
Pfeiffer, Martin J.;Taher, Leila;Boiani, Michele
目前的早期小鼠发育模型将角色分配给随机过程和表观遗传调节，这被认为与小家鼠物种之间存在的遗传差异一样有影响力。本研究的目的是测试小鼠卵母细胞是否在基因产物的丰度方面彼此不同，这些基因产物可能影响、引发甚至预先确定衍生胚胎的发育轨迹和特征。使用近交系小鼠品系的范例，我们定量了在测试的四个品系（129/Sv、C57 BI/6 J、C3 H/HeN、DBA/2 J）的卵母细胞中同时存在的2010个蛋白质组（SILAC LC-MS/MS）和15205个转录物（RNA深度测序）。根据供体品系，卵母细胞在催化和调节蛋白的丰度方面存在差异，通过原位免疫荧光证实了一个子集（邻近锌指结构域的溴结构域，1B [BAZ 1B]，血红素加氧酶1 [HMOX 1]，雌激素相关受体β [ESRRB]）。考虑到Pearson r相关系数为0.18-0.20，不能从同源mRNA的丰度预测卵细胞蛋白的丰度。我们的研究结果表明，一个先决条件，以产生胚胎的多样性，即在卵母细胞中的不同丰度的母体蛋白质，可以在近交系小鼠品系的模型进行研究。因此，我们强调蛋白质组学定量在现代胚胎学中的重要性。所有MS数据均已保存在ProteomeXchange中，标识符为PXD 001059（http：//proteomecentral.proteomexchange.org/soft/PXD 001059）。
Current models of early mouse development assign roles to stochastic processes and epigenetic regulation, which are considered to be as influential as the genetic differences that exist between strains of the species Mus musculus. The aim of this study was to test whether mouse oocytes vary from each other in the abundance of gene products that could influence, prime, or even predetermine developmental trajectories and features of derivative embryos. Using the paradigm of inbred mouse strains, we quantified 2010 protein groups (SILAC LC-MS/MS) and 15205 transcripts (RNA deep sequencing) present simultaneously in oocytes of four strains tested (129/Sv, C57Bl/6J, C3H/HeN, DBA/2J). Oocytes differed according to donor strain in the abundance of catalytic and regulatory proteins, as confirmed for a subset (bromodomain adjacent to zinc finger domain, 1B [BAZ1B], heme oxygenase 1 [HMOX1], estrogen related receptor, beta [ESRRB]) via immunofluorescence in situ. Given a Pearson's r correlation coefficient of 0.18-0.20, the abundance of oocytic proteins could not be predicted from that of cognate mRNAs. Our results document that a prerequisite to generate embryo diversity, namely the different abundances of maternal proteins in oocytes, can be studied in the model of inbred mouse strains. Thus, we highlight the importance of proteomic quantifications in modern embryology. All MS data have been deposited in the ProteomeXchange with identifier PXD001059 (http://proteomecentral.proteomexchange.org/dataset/PXD001059).