PRESERVATION OF TRANSENDOTHELIAL GLUCOSE TRANSPORTER 1 AND P-GLYCOPROTEIN TRANSPORTERS IN A CORTICAL SLICE CULTURE MODEL OF THE BLOOD-BRAIN BARRIER
PRESERVATION OF TRANSENDOTHELIAL GLUCOSE TRANSPORTER 1 AND P-GLYCOPROTEIN TRANSPORTERS IN A CORTICAL SLICE CULTURE MODEL OF THE BLOOD-BRAIN BARRIER
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DOI：
10.1016/j.neuroscience.2010.06.073
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发表时间：
2010-09-29
期刊：
影响因子：
3.3
通讯作者：
Bendfeldt, K.
中科院分区：
文献类型：
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作者：
Camenzind, R. S.;Chip, S.;Bendfeldt, K.
多种神经系统疾病的特征在于血脑屏障（BBB）及其转运蛋白的紊乱。我们最近介绍了成纤维细胞生长因子处理的皮质器官型切片培养的小鼠作为模型，在体外研究的血脑屏障，现在已经进一步表征的维护和功能的转运蛋白通常表达在脑血管内皮。葡萄糖转运蛋白GLUT-1存在于来自出生后第4天至第21天的小鼠在体外培养3天后的切片培养物的血管中。内皮多药耐药性P-糖蛋白（P-gp），这是参与控制药物物质运输通过血脑屏障也保持在血管中，最显着的切片培养物来自出生后第14和21天的小鼠。为了评估P-gp的功能，我们测试了罗丹明123的运输存在或不存在的P-gp抑制剂维拉帕米。在急性切片和体外培养3天的切片中，罗丹明123-荧光在血管腔内迅速积聚。我们的研究结果提供的证据表明，内皮转运蛋白及其功能特性可以保持在器官型皮质切片文化，从而使其成为一个有吸引力的模型系统的血脑屏障的研究。(C)2010年IBRO。由爱思唯尔有限公司出版。保留所有权利。
A variety of neurological diseases are characterized by disturbances of the blood-brain barrier (BBB) and its transporters. We recently introduced fibroblast growth factor treated cortical organotypic slice cultures of mice as a model for in vitro studies of the blood-brain barrier and have now further characterized the maintenance and function of transport-proteins typically expressed in the endothelium of cerebral blood vessels. The glucose transporter GLUT-1 is present in blood vessels of slice cultures derived from postnatal day 4 to 21 mice after 3 days in vitro. The endothelial multidrug resistance P-glycoprotein (P-gp) which is involved in the control of pharmacological substance transport across the blood brain barrier is also maintained in blood vessels, most prominently in slice cultures derived from postnatal day 14 and 21 mice. To assess P-gp function, we tested rhodamine 123 transport in presence or absence of the P-gp inhibitor verapamil. Rhodamine 123-fluorescence accumulated rapidly in the vascular lumen both in acute slices and in slices cultured for 3 days in vitro. Our results provide evidence that endothelial transporters and their functional properties can be maintained in organotypic cortical slices cultures, thus making it an attractive model system for the study of the blood brain barrier. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.