FoxO directly regulates the expression ofTOR/S6Kandvitellogeninto modulate the fecundity of the brown planthopper
FoxO directly regulates the expression ofTOR/S6Kandvitellogeninto modulate the fecundity of the brown planthopper
复制标题
FoxO直接调控TOR/S6K和卵黄原的表达调节褐飞虱的繁殖力
DOI：
10.1007/s11427-019-1734-6
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发表时间：
2020-06-18
影响因子：
9.1
通讯作者：
Zhang, Wenqing
中科院分区：
文献类型：
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作者：
Dong, Yi;Chen, Weiwen;Zhang, Wenqing
FoxO作为一种保守的转录因子，在体内多种生理过程中发挥着至关重要的作用，包括抗逆、长寿、生长和繁殖。以往对FoxO的研究主要集中在人类、小鼠、果蝇和秀丽隐杆线虫上，而对农业害虫的报道很少，而且对FoxO如何调节昆虫繁殖力知之甚少。在亚洲，褐飞虱(BPH)Nilaparvata lugens(Stal)是水稻生产中最严重的害虫之一，其高繁殖力是褐飞虱暴发的基础。在这里，利用 BPH 中 NlFoxO 的全基因组 ChIP-seq，我们发现 NlFoxO 与核糖体蛋白 S6 激酶 (NlS6K) 和丝氨酸/苏氨酸蛋白激酶 mTOR (NlTOR) 的启动子结合并增加其表达水平。我们还发现NlFoxO直接与卵黄原蛋白(NlVg)外显子结合并对其表达具有特异性抑制作用。此外，将NlFoxO双链RNA注射到BPH成虫后，产卵数量和孵化率显着下降。我们的研究结果提供了直接证据，表明 FoxO 通过与 NlS6K、NlTOR 启动子和 NlVg 外显子结合并影响其在 Vg 网络中的基因表达来调节昆虫繁殖力。
As a conserved transcription factor, FoxO plays a crucial role in multiple physiological processesin vivo, including stress resistance, longevity, growth and reproduction. Previous studies on FoxO have focused on human, mouse,Drosophila melanogasterandCaenorhabditis elegans, while there are few reports on agricultural pests and little is known about how FoxO modulates insect fecundity. In Asia, the brown planthopper (BPH)Nilaparvata lugens(Stal) is one of the most serious pests in rice production and high fecundity is the basis of the outbreak of BPH. Here, using the genome-wide ChIP-seq ofNlFoxO in BPH, we found thatNlFoxO binds to the promoters ofribosomal proteinS6 kinase(NlS6K)andserine/threonine-protein kinase mTOR(NlTOR)and increases their expression levels. We also found that NlFoxO directly binds to the exon ofvitellogenin(NlVg)and has a specific inhibitory effect on its expression. In addition, the number of eggs laid and their hatching rate decreased significantly after injection ofNlFoxOdouble-stranded RNA into BPH adults. Our findings provide direct evidence that FoxO modulates insect fecundity through binding to the promoters ofNlS6K, NlTORand the exon ofNlVgand affecting their gene expression in the Vg network.