Influence of Strontium on Vascular Endothelial Growth Factor and Fibroblast Growth Factor 2 Expression in Rat Chondrocytes Cultured In Vitro
Influence of Strontium on Vascular Endothelial Growth Factor and Fibroblast Growth Factor 2 Expression in Rat Chondrocytes Cultured In Vitro
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DOI：
10.1007/s12011-018-1564-y
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发表时间：
2018-11
影响因子：
3.9
通讯作者：
Xiaoyan Zhu;Yezi Kong;Y. Huang;Baoyu Zhao;Jianguo Wang
中科院分区：
文献类型：
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作者：
Xiaoyan Zhu;Yezi Kong;Y. Huang;Baoyu Zhao;Jianguo Wang
锶（Sr）可以减少软骨退变并刺激软骨基质形成。血管生成在软骨形成中起发育作用，并且由生长因子如血管内皮生长因子（VEGF）和成纤维细胞生长因子2（FGF 2）刺激。然而，Sr对软骨细胞中VEGF和FGF 2表达的直接影响尚不完全清楚。本研究的目的是探讨不同浓度的锶对体外培养的大鼠软骨细胞VEGF和FGF 2表达的影响。采用酶消化法从Wistar大鼠关节分离软骨细胞。作为Sr源，将氯化锶六水合物（SrCl 2·6 H2O）以0、0.5、1.0、2.0、5.0、20.0和100.0 μg/mL的最终浓度添加到培养溶液中。连续培养72 h后，分别采用实时荧光定量PCR和Western blot方法检测软骨细胞中VEGF和FGF 2的mRNA丰度和蛋白表达水平。结果显示，软骨细胞VEGF和FGF 2的表达随Sr浓度的增加呈剂量依赖性增加。1.0 μg/mLSr处理组VEGF的mRNA丰度和蛋白表达水平均极显著高于对照组（P< 0.01）。当Sr浓度超过5.0 μg/mL和20.0 μg/mL时，FGF 2的mRNA和蛋白表达与对照组相比差异均有显著性（P< 0.01）。提示Sr可能通过调节VEGF和FGF 2 z的表达参与软骨血管生成。
Strontium (Sr) can reduce cartilage degeneration and stimulate cartilage matrix formation. Angiogenesis plays a developmental role in chondrogenesis, and was stimulated by growth factors such as vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2). However, the direct influence of Sr on VEGF and FGF2 expressions in chondrocytes is not entirely clear. The objective of this study was to investigate the effects of different Sr concentrations on VEGF and FGF2 expressions in rat chondrocytes in vitro. Chondrocytes were isolated from Wistar rat articular by enzymatic digestion. As a Sr source, strontium chloride hexahydrate (SrCl2·6H2O) was added to the culture solution at final concentrations of 0, 0.5, 1.0, 2.0, 5.0, 20.0, and 100.0 μg/mL. After 72 h of continuous culture, mRNA abundance and protein expression levels of VEGF and FGF2 in the chondrocytes were determined by real-time polymerase chain reaction (real-time PCR) and Western blot, respectively. The results showed that VEGF and FGF2 expressions were dose-dependently elevated with Sr concentration in chondrocytes. The mRNA abundance and protein expression levels of VEGF were extremely significantly higher than those in the control group (P< 0.01) at 1.0 μg/mL Sr treatment. For FGF2, there were markedly significant differences in mRNA and protein expression from control group (P< 0.01) when the Sr-treated concentration exceeded 5.0 μg/mL and 20.0 μg/mL, respectively. These results indicated that Sr might involve in the cartilage angiogenesis via regulating expression of VEGF and FGF2z.