Chronic Use of Intracerebral Dialysis for the In Vivo Measurement of 3,4‐Dihydroxyphenylethylamine and Its Metabolite 3,4‐Dihydroxyphenylacetic Acid
Chronic Use of Intracerebral Dialysis for the In Vivo Measurement of 3,4‐Dihydroxyphenylethylamine and Its Metabolite 3,4‐Dihydroxyphenylacetic Acid
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长期使用脑内透析体内测定 3,4-二羟基苯乙胺及其代谢物 3,4-二羟基苯乙酸
作者：
B. Westerink;M. Tuinte
翻译后摘要：脑内透析技术进行了研究，其中大鼠直接连接到HPLC设备的方法。在7天的时间内跟踪三种药物处理[灌注60 mmol K+或5 × 10− 5 M（+）-苯丙胺或皮下注射2 mg/kg（+）-苯丙胺]对3，4-二羟基苯乙胺（多巴胺）和3，4-二羟基苯乙酸释放的影响。在输注K+后观察到的多巴胺输出的显著升高在第3天几乎消失。膜周围的组织反应可能形成了一个屏障，阻止K+到达多巴胺能末梢。相比之下，安非他明（输注以及全身给药）后多巴胺水平的显著升高在植入后8天仍然存在（尽管减少）。它的结论是，有一定的限制，脑透析多巴胺仍然是有用的几天后植入的膜。
Abstract: The intracerebral dialysis technique was studied with a method in which the rat was directly connected to the HPLC equipment. The effect of three pharmacological treatments [perfusion of 60 mmol K+ or 5 × 10−5M (+)‐amphetamine or subcutaneous injection of 2 mg/kg (+)‐amphetamine] on the release of 3,4‐dihydroxy‐phenylethylamine (dopamine) and 3,4‐dihydroxyphenylacetic acid was followed over a period of 7 days. The marked rise of dopamine output seen after infusion of K+ had almost disappeared on day 3. Tissue reactions around the membrane presumably formed a barrier preventing K+ from reaching dopaminergic terminals. In contrast, the pronounced rise in dopamine level after amphetamine (infused as well as systemically administered) was still present (although diminished) 8 days after implantation. It is concluded that, with certain restrictions, brain dialysis of dopamine is still useful several days after implantation of the membrane.