Interaction of chlamydiae with host cells and mucous surfaces.
Interaction of chlamydiae with host cells and mucous surfaces.
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衣原体与宿主细胞和粘膜表面的相互作用。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
S. Ainsworth
中科院分区：
文献类型：
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作者：
J. Pearce;I. Allan;S. Ainsworth
衣原体作为专性细胞内寄生虫，与宿主细胞的附着和摄取是生殖过程中必不可少的步骤。它们的附着位置似乎是热敏感的；它与任何形态实体都没有关联。抗体阻断衣原体与细胞的附着，对于某些对细胞培养高度感染的鹦鹉热衣原体和沙眼衣原体菌株，N-乙酰氨基葡萄糖似乎有助于细胞受体的特异性。唾液酸残基被认为是其他沙眼衣原体菌株的受体。鹦鹉热杆菌豚鼠包涵体结膜炎株在体外接种组织块孵育时与结膜相关。然而，尽管来自泪液的抗体在体内中和了该菌株的感染性，但机体与组织片段的结合并未受到抑制，这表明体内的抗体中和并不是通过阻止与细胞的附着来调节的。通过离心法，衣原体对细胞单层的感染力大大增加。这个过程是温度相关的，涉及方向力和离心力之间的协同作用。感染力的增强似乎是由于细胞表面的变化引起的。这些变化可能有利于附着过程中的非特异性相互作用，因为抗体对静态细胞单层的感染性的抑制可以通过离心法克服。
For chlamydiae, as obligate intracellular parasites, attachment to and ingestion by host cells are essential steps in reproduction. Their attachment site appears to be heat-sensitive; it has not been correlated with any morphological entity. Antibody blocks chlamydial attachment to cells and, for certain chlamydia psittaci and chlamydia trachomatis strains which are highly infective for cell cultures, N-acetylglucosamine appears to contribute to cell receptor specificity. Sialic acid residues have been suggested as receptors for other C. trachomatis strains. The guinea-pig inclusion conjunctivitis strain of C. psittaci becomes associated with the conjunctiva during incubation of inoculated tissue fragments in vitro. However, although antibody from tears neutralizes infectivity of this strain in vivo, association of the organism with tissue fragments is not inhibited, suggesting that antibody neutralization in vivo is not mediated by prevention of attachment to cells. Chlamydial infectivity for cell monolayers is greatly increased by centrifugation. The process is temperature-dependent and involves cooperative interactions between direction force and the pressure generated during centrifugation. Enhanced infectivity appears to result from changes induced in the cell surface. These changes may favour nonspecific interactions in attachment, since antibody inhibition of infectivity on static cell monolayers is overcome by centrifugation.