Effect of dietary omega-3 polyunsaturated fatty acids on experimental periodontitis in the mouse
Effect of dietary omega-3 polyunsaturated fatty acids on experimental periodontitis in the mouse
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DOI：
10.1111/j.1600-0765.2008.01108.x
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发表时间：
2009-04-01
影响因子：
3.5
通讯作者：
Bartold, P. M.
中科院分区：
文献类型：
--
作者：
Bendyk, A.;Marino, V.;Bartold, P. M.
牙周炎是一种主要由革兰氏阴性厌氧菌引起的感染性疾病。宿主对这些细菌的炎症反应导致牙槽骨丢失，这是牙周炎的特征。Omega-3多不饱和脂肪酸具有公认的抗炎作用;其含氧衍生物是减少炎症的关键介质。在这项研究中，我们测试的假设，饮食中补充金枪鱼鱼油丰富的n-3多不饱和脂肪酸，二十二碳六烯酸，将减少牙槽骨丢失的小鼠接种牙周病bacteries.Adult小鼠喂养实验饮食含有10%的金枪鱼油或Sunola油57天。14天后，每种饮食的35只小鼠经口接种牙龈卟啉单胞菌、牙龈卟啉单胞菌和具核梭杆菌的混合物、羧甲基纤维素或保持未处理。处死小鼠，并使用经处理小鼠口腔的软组织活检来测定多不饱和脂肪酸浓度。上颌骨被移除，染色和数字成像，以评估上臼齿周围的骨质流失。与对照组相比，喂食金枪鱼油的小鼠口腔软组织中的n-3多不饱和脂肪酸水平显着较高。给小鼠喂食金枪鱼油并接种牙龈卟啉单胞菌或牙龈卟啉单胞菌和牙龈卟啉单胞菌的组合。与治疗对照组相比，有核体和牙龈卟啉单胞菌分别表现出72%和54%的牙槽骨丢失，牙槽骨丢失与n-3多不饱和脂肪酸组织水平呈负相关。总之，鱼油膳食补充剂作为宿主调节剂可能在预防和/或辅助治疗牙周炎方面具有潜在益处。
Periodontitis is an infective disease caused predominantly by gram-negative anerobes. The host inflammatory response to these bacteria causes alveolar bone loss, which characterizes periodontitis. Omega-3 polyunsaturated fatty acids have recognized anti-inflammatory effects; their oxygenated derivatives are key mediators in reducing inflammation. In this study we tested the hypothesis that dietary supplementation with tuna fish oil rich in the n-3 polyunsaturated fatty acid, docosahexaenoic acid, would reduce alveolar bone loss in mice inoculated with periodontopathic bacteria.Adult mice were fed experimental diets containing either 10% tuna oil or Sunola oil for 57 d. After 14 d, 35 mice on each diet were inoculated orally with Porphyromonas gingivalis, with a mixture of P. gingivalis and Fusobacterium nucleatum, with carboxymethylcellulose or remained untreated. The mice were killed, and soft tissue biopsies from the oral cavity of treated mice were used to determine the polyunsaturated fatty acid concentrations. The maxilla was removed, stained and digitally imaged to assess bone loss around the upper molars.n-3 polyunsaturated fatty acid levels were significantly higher in oral soft tissues of mice fed tuna oil compared with the control group. Mice fed tuna oil and inoculated with P. gingivalis or with the combination of F. nucleatum and P. gingivalis exhibited 72% and 54% less alveolar bone loss respectively, compared with the treatment control group.Alveolar bone loss was inversely related to n-3 polyunsaturated fatty acid tissue levels. In conclusion, fish oil dietary supplementation may have potential benefits as a host modulatory agent in the prevention and/or adjunctive management of periodontitis.