Secondary structure prediction for aligned RNA sequences
Secondary structure prediction for aligned RNA sequences
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DOI：
10.1016/s0022-2836(02)00308-x
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发表时间：
2002-06-21
影响因子：
5.6
通讯作者：
Stadler, PF
中科院分区：
文献类型：
--
作者：
Hofacker, IL;Fekete, M;Stadler, PF
大多数功能性RNA分子在进化过程中具有高度保守的二级结构特征。在这里，我们提出了一种计算一组排列RNA序列的共识结构的方法，同时考虑了热力学稳定性和序列共变。与rnas系统发育结构的比较表明，只有5个相关序列的预测可靠性达到80%以上。作为一项应用，我们表明早期结节mRNA含有重要的二级结构，这是由序列共变支持的。(C) 2002 Elsevier Science Ltd.版权所有。
Most functional RNA molecules have characteristic secondary structures that are highly conserved in evolution. Here we present a method for computing the consensus structure of a set aligned RNA sequences taking into account both thermodynamic stability and sequence covariation. Comparison with phylogenetic structures of rRNAs shows that a reliability of prediction of more than 80% is achieved for only five related sequences. As an application we show that the Early Noduline mRNA contains significant secondary structure that is supported by sequence covariation. (C) 2002 Elsevier Science Ltd. All rights reserved.