A Biomimetic Mussel-Inspired ε-Poly-L-lysine Hydrogel with Robust Tissue-Anchor and Anti-Infection Capacity
A Biomimetic Mussel-Inspired ε-Poly-L-lysine Hydrogel with Robust Tissue-Anchor and Anti-Infection Capacity
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仿生贻贝启发的ε-聚-L-赖氨酸水凝胶，具有坚固的组织锚定和抗感染能力
DOI：
10.1002/adfm.201604894
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发表时间：
2017-02-23
影响因子：
19
通讯作者：
Xu, Hong
中科院分区：
文献类型：
--
作者：
Wang, Rui;Li, Jingzhe;Xu, Hong
原位水凝胶因其侵入性小、能够匹配不规则组织缺损而在组织工程中引起了广泛的关注。然而，多水的生理环境和水凝胶中的高水分严重阻碍了与目标组织的结合，容易引起伤口感染，从而限制了伤口护理管理的有效性。因此，形成水凝胶与组织的亲密组装并防止伤口感染仍然是一个重大挑战。本研究以贻贝黏附蛋白为灵感，利用辣根过氧化物酶交联原位开发了一种仿生多巴胺修饰的epsilon-聚l-赖氨酸-聚乙二醇水凝胶（PPD水凝胶）伤口敷料。PPD聚合物中的仿生儿茶酚-赖氨酸残基分布提供了儿茶酚-赖氨酸的协同效应，使PPD水凝胶具有优异的湿组织粘附性能。结果表明，PPD水凝胶能与生物组织紧密结合，具有良好的体内止血和加速伤口修复能力。此外，由于epsilon-聚l -赖氨酸固有的抗菌能力，水凝胶表现出出色的抗感染性能。这些发现为贻贝启发的组织锚定和抗菌水凝胶材料作为伤口敷料的发展提供了新的线索。
In situ hydrogels have attracted considerable attention in tissue engineering because of their minimal invasiveness and ability to match the irregular tissue defects. However, hydrous physiological environments and the high level of moisture in hydrogels severely hamper binding to the target tissue and easily cause wound infection, thereby limiting the effectiveness in wound care management. Thus, forming an intimate assembly of the hydrogel to the tissue and preventing wound infecting still remains a significant challenge. In this study, inspired by mussel adhesive protein, a biomimetic dopamine-modified epsilon-poly-L-lysine-polyethylene glycol-based hydrogel (PPD hydrogel) wound dressing is developed in situ using horseradish peroxidase cross-linking. The biomimetic catechol-Lys residue distribution in PPD polymer provides a catechol-Lys cooperation effect, which endows the PPD hydrogels with superior wet tissue adhesion properties. It is demonstrated that the PPD hydrogel can facilely and intimately integrate with biological tissue and exhibits superior capacity of in vivo hemostatic and accelerated wound repair. In addition, the hydrogels exhibit outstanding anti-infection property because of the inherent antibacterial ability of epsilon-poly-L-lysine. These findings shed new light on the development of mussel-inspired tissue-anchored and antibacterial hydrogel materials serving as wound dressings.