Recellularization of well-preserved decellularized kidney scaffold using adipose tissue-derived stem cells
Recellularization of well-preserved decellularized kidney scaffold using adipose tissue-derived stem cells
复制标题
使用脂肪组织来源的干细胞对保存良好的脱细胞肾支架进行再细胞化
DOI：
10.1002/jbm.a.36279
复制
发表时间：
2018
影响因子：
4.9
通讯作者：
Zhao Shengtian
中科院分区：
文献类型：
--
作者：
Xue Aibing;Niu Guangzhu;Chen Yuan;Li Kailin;Xiao Zhiying;Luan Yun;Sun Chao;Xie Xiaoshuai;Zhang Denglu;Du Xiaohang;Kong Feng;Guo Yanxia;Zhang Haiyang;Cheng Guanghui;Xin Qian;Guan Yong;Zhao Shengtian
以脂肪组织源性干细胞（ADSC）为种子细胞建立再细胞化肾脏模型，研究ADSC在脱细胞肾脏支架中的生长和分化。使用改进的方法分离ADSC，然后使用流式细胞术分析进行鉴定。进行成骨和成脂分化。使用0.5%十二烷基硫酸钠对大鼠肾脏进行脱细胞。采用免疫荧光、免疫组织化学和扫描电镜观察支架的微观结构。通过动脉顺行或通过输尿管逆行接种ADSC，并培养5-10天。应用苏木精和伊红染色、免疫荧光和免疫组织化学来评估支架内种子细胞的生长和分化。ADSC在肾脏支架的肾小球、血管和肾小管区域内聚集。细胞向内皮细胞或肾小管细胞分化。基质细胞衍生因子1促进支架中的细胞附着。这些发现表明，脂肪干细胞可用作脱细胞肾脏支架内种子细胞的额外新来源。这种组合可能提供供体肾移植的替代方案。通过这种方式，自体ADSC可以用作细胞支架肾再生中的种子细胞，用于进一步的临床移植。© 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part A：106A：805-814，2018.
To establish a recellularization kidney model by using adipose tissue‐derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate. Immunofluorescence, immunohistochemistry, and scanning electron microscope were conducted to examine the scaffold microstructure. The decellularized kidney scaffold was seeded with ADSCs antegrade through the artery or retrograde through the ureter and cultured for 5–10 days. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were applied to assess growth and differentiation of seeding cells within the scaffold. ADSCs populated within the glomerular, vascular, and tubular area of kidney scaffolds. Cells differentiated toward endothelial or tubular cells. Stromal cell‐derived factor 1 promoted cell attachment in the scaffold. These findings suggest that ADSCs can be used as an additional new source of seeding cells within decellularized kidney scaffold. This combination may offer an alternative to donor kidney transplant. In this way, autologous ADSCs can be utilized as seeding cells in cell‐scaffold kidney regeneration for further clinical transplantation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 805–814, 2018.