CNS CELL GROUPS PROJECTING TO PANCREATIC PARASYMPATHETIC PREGANGLIONIC NEURONS
CNS CELL GROUPS PROJECTING TO PANCREATIC PARASYMPATHETIC PREGANGLIONIC NEURONS
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DOI：
10.1016/0006-8993(93)90174-l
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发表时间：
1993-08-27
期刊：
影响因子：
2.9
通讯作者：
HAXHIU, MA
中科院分区：
文献类型：
--
作者：
LOEWY, AD;HAXHIU, MA
用病毒逆行跨神经元标记法鉴定投射到胰腺副交感节前神经元的CNS细胞群。将伪狂犬病病毒(PRV)注射入C8大鼠胰腺，存活6天后灌流，取脑进行免疫组织化学检测。用PRV逆行标记迷走神经背核的副交感节前神经元。几个中枢神经系统细胞组始终含有跨神经元标记的神经元。在延髓孤束核内可见标记神经元。延髓腹侧网状结构中的最后区、三叉神经旁核、巨细胞旁外侧网状核、中缝苍白球和隐核、C3区及散在细胞。在脑桥，A5细胞组、Barrington核和蓝下核内含有标记神经元。臂旁核内仅偶见标记细胞。在中脑，除了中央灰质偶尔有一个神经元外，几乎没有发现任何标记。在间脑，在下丘脑室旁核(PVN)和下丘脑外侧核(PVN和下丘脑底核)均有标记。此外，穹隆周围核和下丘脑背侧核也含有标记神经元。下丘脑背内侧核周边部有少量细胞。下丘脑腹内侧核未见标记。在端脑，杏仁中央核和终纹床核被标记。
The CNS cell groups that project to the pancreatic parasympathetic preganglionic neurons were identified by the viral retrograde transneuronal labeling method. Pseudorabies virus (PRV) was injected into the pancreas of C8 spinal rats and after 6 days survival, the animals were perfused and their brains processed for immunohistochemical detection of PRV. Parasympathetic preganglionic neurons of the dorsal vagal nucleus were retrogradely labeled with PRV. Several CNS cell groups consistently contained transneuronally labeled neurons. In the medulla oblongata, labeled neurons were found in the nucleus tractus solitarius. area postrema, paratrigeminal nucleus, lateral paragigantocellular reticular nucleus, raphe pallidus and obscurus nuclei, C3 region and scattered cells in the ventral medullary reticular formation. In the pons, the A5 cell group, Barrington's nucleus and the subcoeruleus region contained labeled neurons. Only an occasional labeled cell was identified in the parabrachial nucleus. In the midbrain, almost no labeling was found except for an occasional neuron in the central gray matter. In the diencephalon, labeling was found in the paraventricular hypothalamic nucleus (PVN) as well as in the lateral hypothalamic nucleus at two levels (one at the level of the PVN and the other at the level of the subthalamic nucleus). In addition, the perifornical and dorsal hypothalamic nuclei contained labeled neurons. A few cells were found in the peripheral part of the dorsomedial hypothalamic nucleus. No labeling was seen in the ventromedial hypothalamic nucleus. In the telencephalon, the central amygdaloid nucleus and the bed nucleus of the stria terminalis were labeled.