Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria
Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria
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DOI：
10.1007/978-1-4939-6786-5_17
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Diefenbach, Andreas
中科院分区：
文献类型：
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作者：
Gronke, Konrad;Kofoed-Nielsen, Michael;Diefenbach, Andreas
肠粘膜是人体最大的表面积。它不断地受到细菌、共生和致病、原生动物和食物来源刺激物的挑战。为了维持体内平衡，进化出了一个复杂的信号网络，其中包括免疫细胞的作用。近年来，属于先天性免疫系统的淋巴细胞的一个亚群引起了特别的关注。这些所谓的先天淋巴样细胞(ILC)位于小肠和大肠的固有层内，对环境挑战迅速做出反应。它们提供对各种感染的免疫力，但也可能有助于器官的动态平衡，因为它们产生作用于上皮细胞的因子，从而增强屏障的完整性。在这里，我们描述了如何将这些细胞从它们的环境中分离出来，并提供了一个深入的方案，如何通过流式细胞术显示各种ILC亚群。
The intestinal mucosa constitutes the biggest surface area of the body. It is constantly challenged by bacteria, commensal and pathogenic, protozoa, and food-derived irritants. In order to maintain homeostasis, a complex network of signaling circuits has evolved that includes contributions of immune cells. In recent years a subset of lymphocytes, which belong to the innate immune system, has caught particular attention. These so-called innate lymphoid cells (ILC) reside within the lamina propria of the small and large intestines and rapidly respond to environmental challenges. They provide immunity to various types of infections but may also contribute to organ homeostasis as they produce factors acting on epithelial cells thereby enhancing barrier integrity. Here, we describe how these cells can be isolated from their environment and provide an in-depth protocol how to visualize the various ILC subsets by flow cytometry.