Oral Fusobacterium nucleatum subsp. polymorphum binds to human salivary α-amylase.
Oral Fusobacterium nucleatum subsp. polymorphum binds to human salivary α-amylase.
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口腔具核梭杆菌多形亚种与人唾液 α-淀粉酶结合。
DOI：
10.1111/omi.12036
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Takahiko Oho
中科院分区：
文献类型：
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作者：
Maria Zulfiqar;Taihei Yamaguchi;Setsuko Sato;Takahiko Oho
核瘤梭杆菌作为口腔中早期和晚期定殖者之间的中间体。在这项研究中，我们发现具核梭菌多形亚种可以与分子量约为110 kDa的唾液组分结合，并通过飞行时间质谱和免疫反应鉴定了该蛋白质和另一个55 kDa的主要因子，即唾液α-淀粉酶。唾液α-淀粉酶以单体和二聚体形式存在，我们发现二聚体的形成取决于铜离子。核镰孢菌对唾液α-淀粉酶的单体和二聚体都有抑制作用，但与二聚体结合的细菌数量多于与单体结合的细菌数量。不同来源的4种α-淀粉酶对具核梭菌的粘附程度基本相同，而对β-淀粉酶的粘附能力明显降低。在所测试的四种α-淀粉酶抑制剂中，阿卡波糖和来自小麦粉的1型和3型抑制剂显示出显著的抑制具核梭菌与单体和二聚体淀粉酶的粘附的活性，而伏格列波糖几乎没有影响。此外，具核梭菌细胞对唾液α-淀粉酶活性的抑制呈剂量依赖性。这些结果表明，具核梭菌作为口腔微生物定植成熟的早期定植者，发挥着更为重要和积极的作用。
Fusobacterium nucleatumacts as an intermediate between early and late colonizers in the oral cavity. In this study, we showed thatF. nucleatumsubsp.polymorphumcan bind to a salivary component with a molecular weight of approximately 110 kDa and identified the protein and another major factor of 55 kDa, as salivary α‐amylase by time‐of‐flight mass spectrometry and immuno‐reactions. Salivary α‐amylase is present in both monomeric and dimeric forms and we found that formation of the dimer depends on copper ions. TheF. nucleatumadhered to both monomeric and dimeric salivary α‐amylases, but the numbers of bacteria bound to the dimeric form were more than those bound to the monomeric form. The degree of adherence ofF. nucleatumto four α‐amylases from different sources was almost the same, however its binding to β‐amylase was considerably decreased. Among four α‐amylase inhibitors tested, acarbose and type 1 and 3 inhibitors derived from wheat flour showed significant activity against the adhesion ofF.nucleatumto monomeric and dimeric amylases, however voglibose had little effect. MoreoverF. nucleatumcells inhibited the enzymatic activity of salivary α‐amylase in a dose‐dependent manner. These results suggest thatF. nucleatumplays more important and positive role as an early colonizer for maturation of oral microbial colonization.