Deflection of a vibrissa leads to a gradient of strain across mechanoreceptors in a mystacial follicle.
Deflection of a vibrissa leads to a gradient of strain across mechanoreceptors in a mystacial follicle.
复制标题
触须的偏转会导致神秘毛囊中机械感受器的应变梯度。
DOI：
10.1152/jn.00179.2015
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发表时间：
2015
影响因子：
2.5
通讯作者：
Kleinfeld,David
中科院分区：
文献类型：
--
作者：
Whiteley,SamuelJ;Knutsen,PerM;Matthews,DavidW;Kleinfeld,David
啮齿动物使用他们的触须，以检测和歧视触觉功能，在积极的探索。触须感觉运动系统中的机械传导部位是毛囊窦复合体及其相关的触须。我们研究的力学内的环窦（RS）的卵泡在离体制备的小鼠mystyrenepad。窦区有相对密集的默克尔机械感受器和纵向披针形末梢。双光子激光扫描显微镜被用来可视化标记的细胞核在一个100-nl卷之前和之后的触须，这导致在本地化位移的机械感受器细胞的被动偏转，主要是在径向和极方向的触须。这些位移用于计算响应于偏转的穿过毛囊的应变场。我们观察到RS下部区域的压缩，而较低幅度的扩张发生在上部区域，对于10°偏转，体积应变ΔV/V = 0.01。0.1°偏转的外推应变，即据报道由初级神经元引发尖峰的最小角度，对应于激活Piezo 2机械感受器通道的最小应变。
Rodents use their vibrissae to detect and discriminate tactile features during active exploration. The site of mechanical transduction in the vibrissa sensorimotor system is the follicle sinus complex and its associated vibrissa. We study the mechanics within the ring sinus (RS) of the follicle in an ex vivo preparation of the mouse mystacial pad. The sinus region has a relatively dense representation of Merkel mechanoreceptors and longitudinal lanceolate endings. Two-photon laser-scanning microscopy was used to visualize labeled cell nuclei in an ∼100-nl vol before and after passive deflection of a vibrissa, which results in localized displacements of the mechanoreceptor cells, primarily in the radial and polar directions about the vibrissa. These displacements are used to compute the strain field across the follicle in response to the deflection. We observe compression in the lower region of the RS, whereas dilation, with lower magnitude, occurs in the upper region, with volumetric strain ΔV/V ∼ 0.01 for a 10° deflection. The extrapolated strain for a 0.1° deflection, the minimum angle that is reported to initiate a spike by primary neurons, corresponds to the minimum strain that activates Piezo2 mechanoreceptor channels.