Novel Chlamydiales strains isolated from a water treatment plant
Novel Chlamydiales strains isolated from a water treatment plant
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DOI：
10.1111/j.1462-2920.2008.01752.x
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发表时间：
2009-01-01
影响因子：
5.1
通讯作者：
Greub, Gilbert
中科院分区：
文献类型：
--
作者：
Corsaro, Daniele;Feroldi, Veronica;Greub, Gilbert
衣原体是感染自由生活的阿米巴、脊椎动物和一些无脊椎动物的专性胞内细菌。新的成员经常被发现，而且有越来越多的证据支持环境中衣原体的非常重要的多样性。在这项研究中，我们调查了饮用水处理厂中衣原体的存在。将样品接种棘阿米巴单层(共培养)，并将原生阿米巴回收到非营养琼脂上。从棘阿米巴和原生阿米巴混合培养物中用泛衣原体16S rRNA基因聚合酶链式反应搜索衣原体，并通过16S rRNA基因测序确定系统发育类型。通过18S rRNA基因扩增和测序鉴定了原生阿米巴。从总共79个样本中，我们通过棘阿米巴共培养回收了8个衣原体菌株，但28个阿米巴中只有1个含有衣原体。测序结果和系统发育分析表明，我们的菌株属于四个不同的衣原体谱系。4个菌株，包括在其自然寄主内回收的菌株，属于副枝菌科；2个密切相关的菌株属于十字花科；2个不同的菌株与Rhabdochlamydia spp.聚在一起；1个菌株仅与未培养的环境克隆聚在一起。我们的结果证实了阿米巴共培养在从复杂的样品中恢复新的衣原体菌株的有效性，并通过鉴定几个新物种，包括代表一个新家族的第一个菌株，证明了环境中衣原体的巨大多样性。
Chlamydiae are obligate intracellular bacteria infecting free-living amoebae, vertebrates and some invertebrates. Novel members are regularly discovered, and there is accumulating evidence supporting a very important diversity of chlamydiae in the environment. In this study, we investigated the presence of chlamydiae in a drinking water treatment plant. Samples were used to inoculate Acanthamoeba monolayers (Acanthamoeba co-culture), and to recover autochthonous amoebae onto non-nutritive agar. Chlamydiae were searched for by a pan-chlamydia 16S rRNA gene PCR from both Acanthamoeba co-cultures and autochthonous amoebae, and phylotypes determined by 16S rRNA gene sequencing. Autochthonous amoebae also were identified by 18S rRNA gene amplification and sequencing. From a total of 79 samples, we recovered eight chlamydial strains by Acanthamoeba co-culture, but only one of 28 amoebae harboured a chlamydia. Sequencing results and phylogenetic analysis showed our strains belonging to four distinct chlamydial lineages. Four strains, including the strain recovered within its natural host, belonged to the Parachlamydiaceae; two closely related strains belonged to the Criblamydiaceae; two distinct strains clustered with Rhabdochlamydia spp.; one strain clustered only with uncultured environmental clones. Our results confirmed the usefulness of amoeba co-culture to recover novel chlamydial strains from complex samples and demonstrated the huge diversity of chlamydiae in the environment, by identifying several new species including one representing the first strain of a new family.