N-ethylmaleimide sensitization of X-irradiated hypoxic Chinese hamster cells.
N-ethylmaleimide sensitization of X-irradiated hypoxic Chinese hamster cells.
复制标题
X 射线照射缺氧中国仓鼠细胞的 N-乙基马来酰亚胺致敏。
作者：
B. Kimler;W. K. Sinclair;M. Elkind
用氮气和二氧化碳冲洗后，对中国仓鼠细胞进行有氧或低氧X射线照射。与早期数据一致，对于异步电池，氧增强比 (OER) 约为 3。如果在照射期间或照射后立即存在巯基结合剂 N-乙基马来酰亚胺 (NEM)，则主要效果是与未处理的细胞相比，NEM 处理的细胞的存活曲线的外推数显着降低。在缺氧和需氧细胞中观察到了这一点，并且 NEM 处理的细胞的 OER 也约为 3。对于本质上无毒的 NEM 治疗，NEM 与 X 射线协同作用，表明 NEM 抑制细胞修复亚致死损伤的能力。对于有丝分裂选择得到的同步细胞，得到了与上述一致的结果；需要三倍大的剂量才能将缺氧细胞的存活率降低到与需氧细胞相同的水平，无论是否...
Chinese hamster cells were X irradiated either aerobically or hypoxically, after flushing with nitrogen plus carbon dioxide. In agreement with earlier data, for asynchronous cells, the oxygen enhancement ratio (OER) was approximately three. If the sulfhydryl-binding agent N-ethylmaleimide (NEM) was present during or immediately after irradiation, the principal effect was a pronounced decrease in the extrapolation number of the survival curve of NEM-treated cells compared to nontreated cells. This was observed with hypoxic as well as aerobic cells and the OER for NEM-treated cells was also about three. For NEM treatments which were essentially nontoxic, NEM acts synergistically with X rays, suggestive of an inhibition by NEM of a cell's ability to repair sublethal damage. For synchronous cells obtained by mitotic selection, a result consistent with the above was obtained; a dose three times as large was necessary to reduce survival to the same level for hypoxic cells as for aerobic cells, whether or not th...