MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA FOR THE BETA-SUBUNIT OF HUMAN ALCOHOL-DEHYDROGENASE
MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA FOR THE BETA-SUBUNIT OF HUMAN ALCOHOL-DEHYDROGENASE
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DOI：
10.1073/pnas.81.13.4055
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
SMITH, M
中科院分区：
文献类型：
--
作者：
DUESTER, G;HATFIELD, GW;SMITH, M
Human alcohol dehydrogenase (ADH) is encoded by at least 5 genes that fall into 3 classes. The class I ADH genes encode the 3 closely related .alpha., .beta. and .gamma. polypeptides. Molecular genetic analysis of class I ADH genes was initiated by isolating a c[complementary]DNA clone from a human adult liver cDNA library. A synthetic oligonucleotide mixture encoding a portion of the .beta. subunit of ADH was used as an in situ hybridization probe for the cDNA library. One positively hybridizing clone, pADH12, which contained an 1100-base-pair cDNA insert, was subjected to DNA sequence analysis. The cDNA encoded information for the carboxyl-terminal 91 amino acids of a class I ADH and a 3'' untranslated region of 593 nucleotides. Comparisons with the carboxyl terminus of the human ADH .beta. subunit indicated that the cDNA encoded the .beta. polypeptide. This probe may facilitate genetic studies of various human alcohol-related syndromes, as well as enable basic molecular studies on human ADH gene expression.
