Monoclonal antibodies identify four distinct annelid leukocyte markers
Monoclonal antibodies identify four distinct annelid leukocyte markers
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DOI：
10.1016/j.dci.2004.10.008
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Németh, P
中科院分区：
文献类型：
--
作者：
Engelmann, P;Pálinkás, L;Németh, P
本文首次报道了抗赤子爱胜蚓体腔细胞(白细胞)标志物的单抗(抗EFCC克隆)文库的制备和鉴定。白细胞亚群是蚯蚓先天免疫的组成部分，需要使用免疫学标记进行更精确的表征。流式细胞术、免疫细胞化学和免疫沉淀分析证实了抗EFCC克隆的特异性。抗EFCC单抗表现出不同的白细胞亚群和不同的组织染色模式。两种功能分析(如吞噬和包裹)进一步鉴定了EFCC簇，揭示了一个共同的体腔细胞标记和三个亚群特异性标记。在人、小鼠、大鼠和果蝇细胞上未发现交叉反应，而在蜗牛(Planorbarius Kernus)组织中检测到免疫反应。免疫组织化学结果表明，所有的体腔细胞亚群都来自中胚层，这与经典的形态分析相一致。(C)2004爱思唯尔有限公司。保留所有权利。
This paper describes for the first time the production and characterization of a library of monoclonal antibodies (anti-EFCC clones) raised against coelomocyte (leukocyte) markers of Eisenia fetida earthworm. Leukocyte subgroups are components of earthworm innate immunity that require a more precise characterization using immunological markers. Flow cytometry, immunocytochemistry, and immunoprecipitation analyzed and confirmed the specificity of anti-EFCC clones. Anti-EFCC mAbs revealed different leukocyte subpopulations and various staining patterns on tissues. Two functional assays (e.g. phagocytosis and encapsulation) further characterized EFCC clusters revealing a common coelomocyte marker and three subpopulation-specific markers. No crossreactivity was found on human, mouse, rat or cells from Drosophila melanogaster but immunoreactivity was detected on snail (Planorbarius corneus) tissues. Immunohistochemical results suggest mesodermal origin of all coelomocyte subgroups that agree with classical morphological analyses. (c) 2004 Elsevier Ltd. All rights reserved.