Association of the beta isoform of protein kinase C with vimentin filaments.
Association of the beta isoform of protein kinase C with vimentin filaments.
复制标题
蛋白激酶 C β 亚型与波形蛋白丝的关联。
DOI：
10.1002/cm.970220405
复制
发表时间：
1992
影响因子：
--
通讯作者：
Stryer,L
中科院分区：
文献类型：
--
作者：
Spudich,A;Meyer,T;Stryer,L
蛋白激酶C(PKC)亚型是激素、生长因子和神经递质触发的细胞激活途径的关键介质(Nishizuka：Science 233：305-312,1986；Natural 334：661-665,1988)。二酰甘油和钙刺激的激酶活性通常导致PKC从胞浆转移到颗粒组分(Kraft和Anderson：Natural 301：621-623,1983)。在磷脂刺激的大鼠嗜碱性白血病(RBL)细胞中，PKC的p亚型比a亚型移位和降解更快(Huang等人：J.Biol.化学。264：4238-4243,1989)。我们在此报告免疫荧光证据表明，在抗原激活的RBL细胞中，PKC a和p的分布明显不同。PKC-p与核周细丝和从核周区域延伸至细胞周边的细丝结合，而PKC-a则集中在细胞周边区域。这种分布与鬼臼毒素染色和抗微管抗体标记的肌动蛋白细丝和微管明显不同。而PKC p抗体和中间丝蛋白vim entin抗体的染色图谱基本一致，表明PKC p与vim entin细丝结合。这些100A细丝束可能为PKC p与其底物的相互作用提供了对接位置，从而赋予了该亚型作用的特异性。0 1992年Wiley-Liss公司
Protein kinase C (PKC) isoforms are key mediators in hormone, growth factor, and neurotransmitter triggered pathways of cell activation (Nishizuka: Science 233: 305-312, 1986; Nature 334: 661-665, 1988). Stimulation of kinase activity by diacylglycerol and calcium often leads to translocation of PKC from the cytosol to a particulate fraction (Kraft and Anderson: Nature 301: 621-623, 1983). The p isoform of PKC is translocated and degraded much more rapidly than the a isoform in phorbolester-stimulated rat basophilic leukemia (RBL) cells (Huang et al.: J. Biol. Chem. 264: 4238-4243, 1989). We report here immunofluorescence evidence that the distributions of PKC a and p are strikingly different in antigen-activated RBL cells. PKC p associates with perinuclear filaments and filaments that extend from the perinuclear area to the cell periphery whereas PKC a concentrates in regions of the cell periphery. This distribution of PKC p is distinctly different from that of actin filaments and microtubules as determined by phalloidin staining and by anti-tubulin antibody labeling. In contrast, the staining patterns obtained with antibodies to PKC p and to the intermediate filament protein vimentin are almost identical, indicating that PKC p associates with vimentin filaments. These bundles of 100 A filaments may provide docking sites for interactions of PKC p with its substrates and thus confer specificity to the actions of this isoform. 0 1992 Wiley-Liss, Inc.