Early appearance of "natural" mucosal IgA responses and germinal centers in suckling mice developing in the absence of maternal antibodies.
Early appearance of "natural" mucosal IgA responses and germinal centers in suckling mice developing in the absence of maternal antibodies.
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DOI：
10.4049/jimmunol.154.5.2051
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发表时间：
1995-03
影响因子：
4.4
通讯作者：
D. R. Kramer;J. Cebra
中科院分区：
文献类型：
--
作者：
D. R. Kramer;J. Cebra
我们通过比较乳鼠肠道相关淋巴组织（GALT）的免疫状态，研究了被动转移的母体抗体在乳鼠断奶前“自然”粘膜伊加反应的个体发育中的作用。（派伊尔淋巴结，肠系膜淋巴结，和固有层）中，通过C.B17 scid/SCID和正常同类（+/+）成年小鼠。我们还研究了产前与产后转移母体免疫的能力，以防止自然的新生儿粘膜伊加反应的发展，通过交换出生时+/+和scid/scid母亲之间的F1 scid/+幼仔窝。我们的研究结果表明，F1 scid/+幼仔出生或scid/scid母亲的护理经历了自然伊加反应，包括在派尔集合淋巴结和肠系膜淋巴结的生发中心反应的加速发展。这些早期伊加应答是明显的：1）产生IgA的GALT器官培养物的频率增加; 2）GALT器官培养物的平均伊加输出增加; 3）在16日龄时通过ELISPOT检测到的来自GALT的IgA分泌细胞的频率增加（> 1 log）;和4）通过体内溴脱氧尿苷掺入检测到的到17日龄时的生发中心发育。最后，从F1 scid/+幼仔分离的肠道细菌的FACS分析和表面结合的小鼠伊加的存在下染色表明，细菌植物群是一个主要目标的母亲分泌伊加和最早的伊加抗体产生的新生儿GALT的幼仔剥夺了母亲的免疫力。
We have examined the role of passively transferred maternal Abs in the ontogeny of "natural" mucosal IgA responses before weaning of suckling mice by comparing the immune status of gut-associated lymphoid tissue (GALT) (Peyer's patches, mesenteric lymph nodes, and lamina propria) in 7- to 25-day-old F1 severe combined immunodeficient (scid)/+ mice generated through reciprocal crosses of C.B17 scid/scid and normal congenic (+/+) adult mice. We have also examined the ability of prenatal vs postnatal transfer of maternal immunity to forestall the development of natural neonatal mucosal IgA responses by swapping litters of F1 scid/+ pups at birth between +/+ and scid/scid mothers. Our results demonstrate that F1 scid/+ pups born to or nursed by scid/scid mothers undergo an accelerated development of natural IgA responses that include germinal center reactions in both Peyer's patches and mesenteric lymph nodes. These early IgA responses are evident as: 1) increased frequencies of IgA-producing GALT organ cultures; 2) increased mean IgA output by GALT organ cultures; 3) increased frequencies (> 1 log) of IgA-secreting cells from GALT detected by ELISPOT at 16 days of age; and 4) germinal center development by 17 days of age detected by in vivo bromodeoxyuridine incorporation. Finally, FACS analyses of enteric bacteria isolated from F1 scid/+ pups and stained for the presence of surface-bound mouse IgA demonstrate that the bacterial flora is a major target of both maternal secretory IgA and of the earliest IgA Abs produced in the neonatal GALT of pups deprived of maternal immunity.