Correlation between Physicochemical Characteristics of Synthetic Cationic Amphiphiles and Their DNA Transfection Ability.
Correlation between Physicochemical Characteristics of Synthetic Cationic Amphiphiles and Their DNA Transfection Ability.
复制标题
合成阳离子两亲物的理化特性与其 DNA 转染能力的相关性。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
T. Kunitake
中科院分区：
文献类型：
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作者：
T. Akao;T. Osaki;J. Mitoma;A. Ito;T. Kunitake
用合成的双链铵两亲物形成的脂质体用于DNA转染到真核细胞中。我们研究了各种阳离子两亲物在DNA转染中的效率与其理化性质之间的相关性。通过β-半乳糖苷酶cDNA在COS细胞中的瞬时表达来检测两亲物在转染中的效率。通过差示扫描量热法测量，相变温度（Tc）低于37°C的两亲物可以将DNA引入细胞。电子显微镜观察表明，具有DNA转染能力的两亲物在水溶液中形成囊泡结构。因此，流体和囊泡双层结构比刚性和螺旋双层结构的两亲物在DNA转染中的有效性高得多。双十二烷基N-[p-（2-三甲基铵乙氧基）苯甲酰基]-L-谷氨酸溴化物的效率是所有合成的两亲物中最高的。
Liposomes formed with synthetic double-chain ammonium amphiphiles were used for DNA transfection into eukaryotic cells. We studied the correlation between the efficiency of various cationic amphiphiles in DNA transfection and their physicochemical properties. The efficiency of amphiphiles in the transfection was examined by the transient expression of β-galactosidase from its cDNA in COS cells. Amphiphiles with a phase-transition temperature (Tc) lower than 37°C, as measured by differential scanning calorimetry, could introduce DNA into the cells. Electron microscopic observation indicated that amphiphiles possessing DNA transfection ability form vesicular structures in aqueous solution. Thus, fluid and vesicular bilayer structures were much higher than rigid and helical bilayer structures regarding the effectiveness of amphiphiles in DNA transfection. The efficiency of didodecyl N-[p-(2-trimethylammonioethoxy)benzoyl]-L-glutamate bromide was the highest of all the synthetic amphiphiles examined.