Spatial Distribution of Mucilage in the Rhizosphere Measured With Infrared Spectroscopy
Spatial Distribution of Mucilage in the Rhizosphere Measured With Infrared Spectroscopy
复制标题
DOI：
10.3389/fenvs.2018.00087
复制
发表时间：
2018-08-17
影响因子：
4.6
通讯作者：
Carminati, Andrea
中科院分区：
文献类型：
--
作者：
Holz, Maire;Leue, Martin;Carminati, Andrea
由于其对植物生长的影响而受到越来越多的关注，但迄今为止还没有一种方法可以测量其在根际的空间分布。我们测试了是否C-H信号相关的粘液脂肪酸是可检测的红外光谱，如果这种方法可以用来确定在根际粘液的空间分布。玉米植物生长在填充有不含有机物质的土壤的根盒中。红外测量进行了沿着横切垂直以及轴向的根通道。C-H比值的垂直梯度显示C-H值随距离的增加而减小：距牙根中心0.8mm处C-H值接近于零。粘液的测量浓度与以前的研究，这鼓励使用红外光谱定量成像粘液在根际中获得的结果相媲美。
Mucilage is receiving increasing attention because of its putative effects on plant growth, but so far no method is available to measure its spatial distribution in the rhizosphere. We tested whether the C-H signal related to mucilage fatty acids is detectable by infrared spectroscopy and if this method can be used to determine the spatial distribution of mucilage in the rhizosphere. Maize plants were grown in rhizoboxes filled with soil free of organic matter. Infrared measurements were carried out along transects perpendicular as well as axially to the root channels. The perpendicular gradients of the C-H proportions showed a decrease of C-H with increasing distance: 0.8mm apart from the root center the C-H signals achieved a level near zero. The measured concentrations of mucilage were comparable with results obtained in previous studies, which encourages the use of infrared spectroscopy to quantitatively image mucilage in the rhizosphere.