CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays
CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays
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DOI：
10.1182/blood-2002-06-1837
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发表时间：
2003-03-15
期刊：
影响因子：
20.3
通讯作者：
Borrebaeck, CAK
中科院分区：
文献类型：
--
作者：
Högerkorp, CM;Bilke, S;Borrebaeck, CAK
许多研究表明，细胞表面糖蛋白CD44在一些生物学事件中发挥作用，如淋巴生成、归巢、淋巴细胞激活和细胞凋亡。我们早些时候已经报道，除了B细胞受体(BCR)和CD40参与外，幼稚B细胞上的CD44信号还产生了生发中心样表型。为了进一步研究CD44在B细胞分化中的整体作用，我们检测了CD44的表达。体外培养的人B细胞在存在或不存在CD44连接的情况下，以及抗免疫球蛋白(抗Ig)和抗CD40抗体的概况。使用一种新的统计分析方案对来自DNA微阵列的数据集进行分析，该统计分析方案是为了检索CD44连接的最可能的表达模式而创建的。我们的结果表明，CD44结扎可特异性上调IL-6、IL-1α和β(2)-肾上腺素能受体β(2)-AR等基因的表达，提示CD44在免疫调节和炎症中发挥新的作用。
A number of studies have implicated a role for the cell surf ace glyco,protein CD44 in several biologic events, such as lymphopoiesis, homing, lymphocyte activation, and apoptosis. We have earlier reported that signaling via CD44 on naive B cells in addition to B-cell receptor (BCR) and CD40 engagement generated a germinal center-like phenotype. To further characterize the global role of CD44 in B differentiation, we examined the expression. profile of human B cells cultured in vitro in the presence or absence of CD44 ligation, together with anti-immunoglobulin (anti-Ig) and anti-CD40 antibodies. The data sets derived from DNA microarrays Were analyzed using a novel statistical analysis scheme created to retrieve the most likely expression pattern of CD44 ligation. Our results show that genes such as interleukin-6 (IL-6), IL-1alpha, and beta(2)-adrenergic receptor beta(2)-AR) were specifically up-regulated by CD44 ligation, suggesting a novel role for CD44 in immunoregulation and inflammation.