Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants
Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants
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瘤胃酸中毒绵羊模型瘤胃液移植后瘤胃发酵和细菌群落的动态变化：对反刍动物瘤胃健康的影响。
DOI：
10.1096/fj.201802456r
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发表时间：
2019-07-01
期刊：
影响因子：
4.8
通讯作者：
Mao, Shengyong
中科院分区：
文献类型：
--
作者：
Liu, Junhua;Li, Hongwei;Mao, Shengyong
近年来，瘤胃液移植（RT）已成功地应用于治疗反刍动物急性瘤胃酸中毒，但其对瘤胃微生物稳态和宿主功能的影响尚不清楚。本研究采用绵羊瘤胃酸中毒模型，研究了RT后瘤胃发酵和细菌群落的动态变化及其对瘤胃上皮细胞形态和功能的有益影响。结果表明，RT引起瘤胃发酵的动态变化，提高了瘤胃内总挥发性脂肪酸、乙酸、丙酸和丁酸的含量，降低了乳酸和LPS的含量。Illumina MiSeq测序数据显示，RT促进了瘤胃细菌稳态的快速重建（对照中为8天，RT中为2天），从明显的生态失调性酸中毒状态恢复到健康水平（与供体的水平相似）。在属的水平上，RT增加了未分类的拟杆菌目，未分类的普雷沃氏菌科，未分类的瘤胃球菌科和Acetitomaculum的相对丰度。此外，RT还加速了预测的瘤胃细菌宏基因组功能的恢复。瘤胃乳头形态学观察结果表明，RT能减轻急性瘤胃酸中毒对瘤胃上皮细胞的损伤，增加瘤胃乳头长度。此外，实时荧光定量PCR结果表明，RT调控细胞因子和紧密连接相关基因的mRNA表达在瘤胃上皮细胞。综上所述，这些结果表明RT加速了瘤胃发酵和细菌稳态的恢复，并调节了瘤胃上皮细胞的形态和功能。刘杰，Li，H.，朱伟，Mao，S.瘤胃酸中毒绵羊模型中瘤胃液移植后瘤胃发酵和细菌群落的动态变化：对反刍动物瘤胃健康的意义。
Over the years, rumen fluid transplantation (RT) has been successfully applied to treat acute rumen acidosis in ruminants, but how it functions in the ruminal microbial homeostasis and host function remains largely unknown. Here, we investigated the dynamic changes of rumen fermentation and bacterial communities following RT and its beneficial effects on rumen epithelial morphology and function in a sheep model of rumen acidosis. The results showed that RT resulted in dynamic changes in rumen fermentation and increased the concentrations of total volatile fatty acid, acetate, propionate, and butyrate, but it decreased the levels of lactate and LPS in the rumen. Illumina MiSeq Sequencing data showed that RT facilitated rapid rebuilt of ruminal bacterial homeostasis (8 d in control vs. 2 d in RT) from a markedly dysbiotic acidosis state to a healthy level (similar with those of donors). At the genus level, RT increased the relative abundance of unclassified Bacteroidales, unclassified Prevotellaceae, unclassified Ruminococcaceae, and Acetitomaculum. Additionally, RT also accelerated recovery of the predicted metagenomic function of ruminal bacteria. Rumen papillae morphology results showed that RT alleviated the damage of rumen epithelia induced by acute rumen acidosis and increased the length of rumen papillae. Furthermore, real-time PCR results showed that RT modulated mRNA expression of genes related to cytokines and tight junctions in the rumen epithelia. In summary, these results reveal that RT accelerates recovery of rumen fermentation and bacterial homeostasis and modulates rumen epithelial morphology and function for sheep suffering from rumen acidosis.-Liu, J., Li, H., Zhu, W., Mao, S. Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants.