Intramuscular injection of expression plasmid DNA is an effective means of long-term systemic delivery of interleukin-5.
Intramuscular injection of expression plasmid DNA is an effective means of long-term systemic delivery of interleukin-5.
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肌肉注射表达质粒DNA是长期全身输送IL-5的有效手段。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Jun
中科院分区：
文献类型：
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作者：
M. Tokui;Izumi Takei;Fumi Tashiro;Akira Shimada;Akira Kasuga;M. Ishii;Toshiharu Ishii;Kiyoshi Takatsu;Takao Saruta;Jun
已经证明肌内注射表达质粒DNA被肌纤维吸收并随后表达外源基因。在本研究中，我们评估了肌内DNA注射作为全身递送白介素-5 （IL-5）的一种手段。在CAG启动子的调控下，构建了含有小鼠IL-5 cDNA的IL-5表达质粒pCAGGS-IL-5。用布比卡因预处理小鼠比目鱼肌。两天后，在同一部位注射pCAGGS- il -5或对照pCAGGS质粒DNA。注射DNA 2周后，10只注射pCAGGS- il -5的小鼠中有9只外周血白细胞的嗜酸性粒细胞从2-3%增加到8-29%，而注射pCAGGS的对照组小鼠的嗜酸性粒细胞从未超过3%。注射pCAGGS-IL-5后，肌区存在IL-5 mRNA。在注射pCAGGS-IL-5的大多数小鼠血清中，ELISA也检测到IL-5，但在对照组小鼠中没有检测到IL-5。这些结果表明，肌内质粒注射通过结合强CAG启动子和布比卡因预处理，可以作为一种有效的全身递送细胞因子的方法。
It has been demonstrated that intramuscularly injected expression plasmid DNA is taken up by myofibers and subsequently expresses exogenous genes. In the present study, we assessed intramuscular DNA injection as a means of systemically delivering interleukin-5 (IL-5). We constructed an IL-5 expression plasmid, pCAGGS-IL-5, containing murine IL-5 cDNA under the control of the CAG promoter. The soleus muscle of mice was pretreated with bupivacaine. Two days later, mice were injected with pCAGGS-IL-5 or a control pCAGGS plasmid DNA at the same site. At 2 weeks after DNA injection, eosinophils had increased from 2-3% to 8-29% of peripheral white blood cells in 9 of 10 mice injected with pCAGGS-IL-5, while eosinophils never exceeded 3% in control mice injected with pCAGGS. IL-5 mRNA was present in the muscle area injected with pCAGGS-IL-5. IL-5 was also detectable by ELISA in the sera of most mice injected with pCAGGS-IL-5, but in none of the control mice. These results demonstrate that intramuscular plasmid injection serves as a useful method of systemically delivering cytokines by combining the strong CAG promoter and bupivacaine pretreatment.
影响因子：
3.5
作者：
Wolff, Jon A.;Ludtke, James J.;Jani, Agnes
通讯作者：
Jani, Agnes