Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding
Isolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding
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DOI：
10.4269/ajtmh.2002.66.225
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发表时间：
2002-03-01
影响因子：
3.3
通讯作者：
Godfroid, E
中科院分区：
文献类型：
--
作者：
Leboulle, G;Rochez, C;Godfroid, E
在扁虱的唾液腺中，吸血过程中诱导的基因会导致分泌到扁虱唾液中的新蛋白质的表达。这些蛋白质可能参与调节脊椎动物的宿主免疫和止血反应。本研究利用未投喂和暴食5日龄的蓖麻硬蜱唾液腺中提取的mRNA，构建了消减文库和全长文库。将这两个文库的序列与欧洲分子生物学实验室(EMBL)/GenBank数据库进行比对，将其分为两大类。第一组包括与已知功能基因不匹配或同源性较低的cDNA。第二组包括与已知功能的基因高度同源的序列--例如，抗凝剂、血小板聚集抑制物和免疫调节蛋白。对相应蛋白质的分析表明，它们可能是由唾液腺细胞分泌的。为了研究重组蛋白的性质，选择了在哺乳动物或细菌系统中表达的cDNA。
In tick salivary glands, genes induced during blood feeding result in the expression of new proteins secreted into tick saliva. These proteins are potentially involved in modulation of vertebrate host immune and hemostatic responses. In this study, subtractive and full-length cDNA libraries were constructed by use of mRNA extracted from salivary glands of unfed and 5-day engorged Ixodes ricinus. Sequences from these 2 libraries were compared with European Molecular Biology Laboratory (EMBL)/GenBank databases which led to their classification into 2 major groups. The first group comprises cDNAs that failed to match or showed low homology to genes of known function. The second group includes sequences that showed high homology to genes of known function-for example, anticoagulants, inhibitors of platelet aggregation, and immunomodulatory proteins. Analyses of corresponding proteins suggest that they may be secreted by salivary gland cells. To study the properties of the recombinant proteins, selected cDNAs were expressed in mammalian or bacterial systems.