Extracellular Iodophilic Polysaccharide Synthesized by Neisseria in Human Dental Plaque

Extracellular Iodophilic Polysaccharide Synthesized by Neisseria in Human Dental Plaque
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人牙菌斑中奈瑟菌合成的细胞外亲碘多糖

DOI:
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发表时间:
1982
期刊:
Journal of dentistry research
影响因子:
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通讯作者:
D.A. Stelzig
D.A. Stelzig
中科院分区:
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文献类型:
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作者:
J. Ruby;R. Shirey;V.F. Gerencser;D.A. Stelzig

文献摘要

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从儿童前牙列唇侧采集的牙菌斑中含有奈瑟氏菌,该菌可从蔗糖中合成细胞外嗜碘多糖(EIP)。完全乙酰化的EIP衍生物的气相色谱图表明,葡萄糖(97%)和半乳糖(3%)是唯一的单糖成分存在。EIP的部分甲基化的糖醇乙酸酯(PMAA)衍生物的表征表明,它主要是线性1,4葡聚糖(75.5%),与一些1,6葡聚糖(8.4%)。与直链淀粉和糖原相比,EIP的相对线性(96.6%)通过分光光度法吸收研究得到证实。
Dental plaque collected from the labial aspect of the anterior dentition of children harbored Neisseria that synthesized an extracellular iodophilic polysaccharide (EIP) from sucrose. Gas chromatograms of fully-acetylated EIP derivatives indicated that glucose (97%) and galactose (3%) were the only monosaccharide constituents present. The characterization of the partially-methylated alditol acetate (PMAA) derivatives of EIP indicated that it was primarily a linear 1,4 glucan (75.5%), with some 1,6 glucan (8.4%). The relative linearity of EIP (96.6%) as compared to that of amylose and glycogen was confirmed by spectrophotometric absorption studies.