Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex
Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex
复制标题
视觉皮层兴奋性神经元核形状动力学与年龄相关的减少
DOI：
10.1101/2022.08.22.504704
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yusuke Kishi
中科院分区：
文献类型：
--
作者：
Tanita Frey;Tomonari Murakami;Koichiro Maki;Takumi Kawaue;Ayaka Sugai;Naotaka Nakazawa;Taiji Adachi;Mineko Kengaku;Kenichi Ohki;Yukiko Gotoh;Yusuke Kishi
神经元的功能随着时间的推移而下降，年龄相关的神经元变化与神经退行性疾病的表型相关。在非神经组织中，内折核形状已被提出作为老化细胞的标志，并且具有内折核的神经元也已被报道与神经元活动相关。在这里，我们对Nex-Cre; SUN 1-GFP小鼠的视觉皮质进行了延时成像。在年轻细胞核中，在刺激10分钟内观察到细胞核内折叠，而老化细胞核在刺激前已经内折叠，并显示出形态学的动力学降低。在年轻的核中，耗尽的刺激恢复核的球形和减少的动力学行为，这表明核折叠是一个可逆的过程。我们还发现老年细胞核比年轻细胞核更硬，这与年龄相关的核形状动力学损失有关。我们揭示了外部刺激后的核形状的时间变化，并观察到这些形态动态随年龄的增长而下降。
Neurons decline in their functionality over time, and age‐related neuronal alterations are associated with phenotypes of neurodegenerative diseases. In nonneural tissues, an infolded nuclear shape has been proposed as a hallmark of aged cells and neurons with infolded nuclei have also been reported to be associated with neuronal activity. Here, we performed time‐lapse imaging in the visual cortex ofNex‐Cre;SUN1‐GFPmice. Nuclear infolding was observed within 10 min of stimulation in young nuclei, while the aged nuclei were already infolded pre‐stimulation and showed reduced dynamics of the morphology. In young nuclei, the depletion of the stimuli restored the nucleus to a spherical shape and reduced the dynamic behavior, suggesting that nuclear infolding is a reversible process. We also found the aged nucleus to be stiffer than the young one, further relating to the age‐associated loss of nuclear shape dynamics. We reveal temporal changes in the nuclear shape upon external stimulation and observe that these morphological dynamics decrease with age.
Polycomb 组蛋白 Ring1 调节小鼠端脑的背腹侧模式
DOI：
10.1101/639492
发表时间：
2019
期刊：
bioRxiv
影响因子：
--
作者：
Eto Hikaru;Kishi Yusuke;Koseki Haruhiko;Gotoh Yukiko
通讯作者：
Gotoh Yukiko
影响因子：
2.4
作者：
Newberg J;Schimpf J;Woods K;Loisate S;Davis PH;Uzer G
通讯作者：
Uzer G
影响因子：
3.5
作者：
BUTT, HJ;JASCHKE, M
通讯作者：
JASCHKE, M