Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
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DOI：
10.1007/s00248-012-0073-3
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发表时间：
2012-11-01
影响因子：
3.6
通讯作者：
Elshahed, Mostafa S.
中科院分区：
文献类型：
--
作者：
Davis, James P.;Struchtemeyer, Christopher G.;Elshahed, Mostafa S.
我们监测的气水分离器和储水罐中的两个新钻的天然气威尔斯井在巴内特页岩在德克萨斯州中北部的细菌群落，使用16 S rRNA基因焦磷酸测序方法超过6个月的时间。总体而言，社区主要由中度嗜盐和耐盐成员的厚壁菌门和变形菌门（类I 'eta-，伽马-，和Epsilonproteobacteria）在两个威尔斯在所有采样时间和位置。许多观察到的谱系是在以前对来自各种化石流体地层和生产设施的微生物群落的调查中遇到的。在所有的样品中，遇到了多个产生H2S的谱系;属于硫酸盐和硫还原类Deltaproteobacteria，梭菌目和协同门，以及硫代硫酸盐还原目的Halanaerobiales。来自分离器和罐样品的细菌群落与用于钻探这些威尔斯井的钻井泥浆和水力压裂沃茨中的细菌群落几乎没有相似之处，这表明这些井组分中独特细菌群落的原位发育是对当前普遍的地球化学条件的响应。相反，在时间和空间尺度上的细菌群落的比较表明，在每个采样位置的核心微生物群落的建立。结果提供了第一个概述的细菌动力学和定植模式，在新钻的，产热的天然气威尔斯井和突出的空间和时间的变化模式观察到的细菌群落在天然气生产设施。
We monitored the bacterial communities in the gas-water separator and water storage tank of two newly drilled natural gas wells in the Barnett Shale in north central Texas, using a 16S rRNA gene pyrosequencing approach over a period of 6 months. Overall, the communities were composed mainly of moderately halophilic and halotolerant members of the phyla Firmicutes and Proteobacteria (classes I'eta-, Gamma-, and Epsilonproteobacteria) in both wells at all sampling times and locations. Many of the observed lineages were encountered in prior investigations of microbial communities from various fossil fluid formations and production facilities. In all of the samples, multiple H2S-producing lineages were encountered; belonging to the sulfate- and sulfur-reducing class Deltaproteobacteria, order Clostridiales, and phylum Synergistetes, as well as the thiosulfate-reducing order Halanaerobiales. The bacterial communities from the separator and tank samples bore little resemblance to the bacterial communities in the drilling mud and hydraulic-fracture waters that were used to drill these wells, suggesting the in situ development of the unique bacterial communities in such well components was in response to the prevalent geochemical conditions present. Conversely, comparison of the bacterial communities on temporal and spatial scales suggested the establishment of a core microbial community in each sampled location. The results provide the first overview of bacterial dynamics and colonization patterns in newly drilled, thermogenic natural gas wells and highlights patterns of spatial and temporal variability observed in bacterial communities in natural gas production facilities.