Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC.
Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC.
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DOI：
10.1016/j.talanta.2003.11.016
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发表时间：
2004-05
期刊：
影响因子：
6.1
通讯作者：
Laisheng Li;Min Liu;S. Da;Yuqi Feng
中科院分区：
文献类型：
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作者：
Laisheng Li;Min Liu;S. Da;Yuqi Feng
采用高效液相色谱法研究了核苷类、嘧啶类和嘌呤类在新型对叔丁基杯[8]芳烃键合硅胶固定相（CABS）上的色谱行为。比较了它们在ODS上的滞留行为。研究了甲醇含量、pH、离子强度等流动相变量对保留行为的影响。CABS上的一些核苷、嘧啶和嘌呤被成功分离。结果表明，杯状[8]芳烃键合相在高水流动相中对上述分析物具有较高的选择性。色谱数据表明，疏水相互作用、氢键相互作用和偶极子-偶极子相互作用是其保留行为的主要原因。此外，在一定程度上，分析物在CABS上的垂直堆积作用也会改变其保留行为。CABS在常规快速分离核苷和碱基方面优于ODS。
The chromatographic behavior of some nucleosides, pyrimidines and purines on a new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) were studied by high performance liquid chromatography. Their retention behavior on CABS were compared with those on ODS. The influence of mobile phase variables, such as methanol content, pH and ionic strength on the retention behavior were studied. Some nucleosides, pyrimidines and purines on CABS were successfully separated. The results show that the calix[8]arene-bonded phase exhibits high selectivities for the above analytes in high aqueous mobile phases. According to the chromatographic data, it is indicated that hydrophobic interaction, hydrogen-bonding interaction, and dipole–dipole interaction are mainly responsible for the retention behavior. In addition, in some extent, the vertical stacking action of the analytes on CABS can also change the retention behavior. CABS was superior to ODS in the routine fast separation of nucleosides and bases.